畜牧兽医学报2024，Vol.55Issue(5)：2042-2049,8.DOI:10.11843/j.issn.0366-6964.2024.05.022
饲粮添加赖氨酸对肉牛粪便发酵参数和微生物菌群结构的影响
Effects of Dietary Lysine Supplementation on Fecal Fermentation Parameters and Microbial Flora Structure of Beef Cattle
摘要
Abstract
This study was conducted to investigate the effects of dietary lysine supplementation on fecal fermentation parameters,microbial diversity and microflora structure of beef cattle.Four healthy Jinjiang cattle with similar body weight were randomly divided into two groups with two cattle in each group.A repeated 2×2 Latin square design was adopted with the control group fed the basal diet and the treatment group fed the basal diet+0.20％of lysine.The experiment lasted for 30 days and was divided into 2 phases,in which the first 10 days of each phase designated as the adaptation period and the last 5 days being the sample collection period.Fecal samples were collected and then analyzed for fermentation parameters,microbial diversity and microflora structure.The results showed as follows:1)Dietary supplementation of lysine significantly increased the concentrations of isobutyrate,isovalerate and branched-chain volatile fatty acids in feces(P＜0.05);2)Lysine supplementation had no significant effect on fecal microbial richness and evenness(P＞0.05);3)Taxonomic annotation found that the supplementation of lysine significantly increased the relative abundance of Lachnospiraceae NK 4A136 group in feces,whilst the relative abundance of Christensenellaceae R-7 group was decreased as compared to the control group(P＜0.05);4)Both principal coordinate analysis(PCoA)and analysis of similarity(ANOSIM)showed that lysine supplementation had no significant effect on fecal microbial community structure.In conclusion,dietary supplementation of 0.20％lysine could improve the concentrations of isobutyrate,isovalerate and branched-chain volatile fatty acids in feces,as well as alterations in the relative abundances of Lachnospiraceae NK4A136 group and Christensenellaceae R-7 group,but has no significant effect on fecal microbial diversity and microflora structure.关键词
赖氨酸/粪便/发酵参数/微生物多样性/菌群结构Key words
lysine/feces/fermentation parameter/microbial diversity/microflora structure分类
农业科技引用本文复制引用
龙唐晖,詹彦波,廖观香,陈新锋,张健,李艳娇,欧阳克蕙,邱清华..饲粮添加赖氨酸对肉牛粪便发酵参数和微生物菌群结构的影响[J].畜牧兽医学报,2024,55(5):2042-2049,8.基金项目
国家自然科学基金项目(32260861 （）
32160807) （）
江西省自然科学基金资助项目(20232BAB215051) （20232BAB215051）
江西省主要学科学术和技术带头人培养计划"领军人才"项目(20213BCJL22043) （20213BCJL22043）
江西农业大学年轻科研创新团队项目(JXAUCXTD008) （JXAUCXTD008）
