基于谱效关系和网络药理学的穿心莲抗氧化物质基础研究OA北大核心CSTPCD

This study was to explore the HPLC fingerprint of Andrographis paniculata and the relationship between its spectrum and efficacy in vitro,to construct a network of the antioxidant effect of Andrographis paniculata,and to integrate the spectrum and the network pharmacol-ogy information,for purpose of predicting the material basis of oxidation resistance of Andrographis paniculatafor.The fingerprints of 10 bat-ches of Andrographis paniculata were established by HPLC,and the contents of ROS and MDA in BRL-3A cells treated with Andrographis paniculate were determined using a microplate reader,and the antioxidant ingredients were preliminarily determined by grey correlation analy-sis.The candidate components of antioxidants were predicted in combination of network pharmacology in order to analyze the antioxidant com-ponents of Andrographis paniculata.The results were that the fingerprint of Andrographis paniculata was established and 11 common peaks were calibrated.The grey correlation analysis showed that the common peaks of 9,7,10,5,1,6,8 and 3 had a higher relevance with the ROS content of BRL-3A cells,and that the common peaks of 9,10,5,7,6,8,1 and 3 had a higher relevance with the MDA content of BRL-3A cells.According to the network pharmacology,25 components,including 14-deoxyandrographolide,neo-andrographolide,and rographidine A,were speculated to be the antioxidant components of Andrographis paniculata.The integrated analysis suggested that the an-tioxidant components of Andrographis paniculata were 9,7,10,5,1,6,8 and 3 common peaks,and 14-deoxyandrographolide,neo-an-drographolide,and so on.Taken together,the antioxidant effect of Andrographis paniculatais was believed to be attributed to the interaction of various components.The active ingredients of Andrographis paniculata might exert their pharmacological effects mainly through the apoptotic pathway,acting on AKT1,EGFR,CASP3 and other targets.The results provided reference for future research on the material basis of the antioxidant effect of Andrographis paniculata.