中国病理生理杂志2024，Vol.40Issue(5)：899-907,9.DOI:10.3969/j.issn.1000-4718.2024.05.015
白杨素减轻非酒精性脂肪性肝炎小鼠脂肪变性及血脂异常
Chrysin attenuates hepatic steatosis and blood lipid dysregulation in a mouse model of nonalcoholic steatohepatitis
摘要
Abstract
AIM:To investigate the therapeutic effects of chrysin on nonalcoholic steatohepatitis(NASH).METHODS:Eight-week-old male C57BL/6 mice were randomly divided into control group,model group,and chrysin group.The mice in control group were fed with normal diet,and those in model and chrysin groups were fed with methio-nine-and choline-deficient(MCD)diet.After 5 weeks of adaptation,the mice in chrysin group received chrysin treatment(20 mg/kg)by continuous lavage for 6 weeks,while those in control and model groups were given equal volume of saline.During the experiment,the health condition of the mice was monitored.Liver morphology was examined after the mice were sacrificed.Serum triglyceride(TG),total cholesterol(TC),low-density lipoprotein cholesterol(LDL-C),high-den-sity lipoprotein cholesterol(HDL-C),alanine aminotransferase(ALT)and aspartate aminotransferase(AST)levels were measured using a biochemical analyzer.Liver tissue TG and TC levels were measured using assay kits.Liver cell damage and inflammation were assessed by hematoxylin-eosin(HE)staining and F4/80 immunohistochemistry staining.The ex-tent of liver lipid deposition was explored by oil red O staining.Masson staining and Sirius red staining were performed to assess liver fibrosis.Immunohistochemistry was performed to analyze the expression of fibrosis-related molecules.RE-SULTS:Compared with control group,the mice in model group showed significant decrease in body weight,liver wet weight,and liver volume.Serum TG,LDL-C,ALT and AST levels,as well as liver TG and TC levels were significantly elevated,and HDL-C levels were decreased in model group.Pathological staining showed significant inflammatory cell in-filtration,lipid deposition,and liver fibrosis.After the treatment with chrysin,increased body weight and liver weight,a reddish appearance of the liver,relatively smooth surface,and sharp liver edges were observed.Serum TG,LDL-C,AST and ALT levels,and liver TG levels were significantly reduced by chrysin.Inflammatory cell infiltration,lipid deposition,and liver tissue fibrosis were also significantly attenuated by chrysin.CONCLUSION:Chrysin shows a potential as a can-didate drug for the treatment of NASH by inhibiting hepatic steatosis,inflammation,and liver fibrosis.关键词
白杨素/非酒精性脂肪性肝炎/蛋氨酸胆碱缺乏饮食/肝脏纤维化Key words
chrysin/nonalcoholic steatohepatitis/methionine-and choline-deficient diet/liver fibrosis分类
医药卫生引用本文复制引用
王星星,李沫,高川月,熊伯成,张秀芬,李晓梅,杨细飞..白杨素减轻非酒精性脂肪性肝炎小鼠脂肪变性及血脂异常[J].中国病理生理杂志,2024,40(5):899-907,9.基金项目
国家自然科学基金资助项目(No.82171583) （No.82171583）
深圳市医学重点学科建设经费资助项目(No.SZXK069) （No.SZXK069）
深圳市科技创新委员会重点基础研究计划项目(No.JCYJ20200109150717745XY （）
No.JCYJ20200109144418639) （）
深圳市医疗卫生三名工程项目(No.SZSM201611090) （No.SZSM201611090）
