实验室非洲猪瘟病毒感染途径与感染剂量研究现状OA

African swine fever virus(ASFV)could be transmitted through direct and indirect contact with infected pigs via various routes,with a complex pathogenic mechanism.In researches on ASFV,and particularly in vaccine development,the lack of international standards for infection routes and doses hampered further step of researches,to this end,domestic and international researches on ASFV infection routes and doses were reviewed.According to the available studies,major infection routes included oral infection,nasal infection,oral-nasal infection,simulated cohabitation infection and intramuscular injection infection,among which oral-nasal vaccination was commonly used.However,because of too many uncertainties associated with oral-nasal vaccination,most current researches,especially those evaluating the safety and efficacy of vaccines,were carried out through intramuscular injection,only a very few doses could cause infection.In the researches on ASFV infection,different challenge doses were set up by researchers to evaluate the safety and efficacy of vaccines for selecting an appropriate dose to better simulate clinical infection,so as to obtain true and reasonable experimental data and subsequently reliable conclusions.