一株多重耐药大肠杆菌全基因组测序及其耐药性分析OA北大核心CSTPCD

In order to understand colistin resistance genes carried by Escherichia coli,screen sensitive plant drugs,and solve the dilemma of multiple drug resistance and no drug option in animal clinic,the resistance genes of Escherichia coli were identified by 16S rRNA sequencing and PCR.The antimicrobial susceptibility of 78 antibiotics were detected,and the bacteriostatic and bactericidal effects of 4 kinds of natural plant extracts(palmatine,berberine,baicalin and macleaya cordata)were tested.The results showed that 145 strains of E.coli were isolated and identified from intestinal tract of swine clinical diarrhea cases in 2021(from January to December)and 2022(from January to June),and a clinical strain carrying colistin resistance genes(mcr-4,mcr-5)and β-lactamases blaTEM and AmpC was identified,which named HN2149.The antimicrobial susceptibility of 78 antibiotics showed that the HN2149 strains was sensitive to cefepime,cefodizime,fosfomycin,cefixime,meropenem,cefoxitin,cefazolin,cefoperazone,cefotaxime,ceftazidime,cefuroxime,ceftriaxone,aztreonam,piperacillin-tazobactam,ticacillin/clavulanic acid,cefoperazone sulbactam,ceftazidime/clavulanic acid,cefotaxime/clavulanic acid,ceftizoxime,cefmetazole,cefetamet,and was resistant to 57 antibiotics.The drug sensitivity of 4 plant extracts showed that the macleaya cordata had the best bacteriostatic and bactericidal effect on HN2149 strain,while the other 3 extracts had no effects on HN2149 strain.Above results provided reference for the prevention and control of swine colibacillosis.