|国家科技期刊平台
首页|期刊导航|药物分析学报(英文)|Gut microbiome-based thiamine metabolism contributes to the protective effect of one acidic polysaccharide from Selaginella uncinata(Desv.)Spring against inflammatory bowel disease

Gut microbiome-based thiamine metabolism contributes to the protective effect of one acidic polysaccharide from Selaginella uncinata(Desv.)Spring against inflammatory bowel diseaseOA

Gut microbiome-based thiamine metabolism contributes to the protective effect of one acidic polysaccharide from Selaginella uncinata(Desv.)Spring against inflammatory bowel disease

英文摘要

Inflammatory bowel disease(IBD)is a serious disorder,and exploration of active compounds to treat it is necessary.An acidic polysaccharide named SUSP-4 was purified from Selaginella uncinata(Desv.)Spring,which contained galacturonic acid,galactose,xylose,arabinose,and rhamnose with the main chain structure of →4)-α-D-GalAp-(1 → and →6)-β-D-Galp-(1 → and the branched structure of →5)-α-L-Araf-(1 →.Animal experiments showed that compared with Model group,SUSP-4 significantly improved body weight status,disease activity index(DAI),colonic shortening,and histopathological damage,and elevated occludin and zonula occludens protein 1(ZO-1)expression in mice induced by dextran sulfate sodium salt(DSS).16S ribosomal RNA(rRNA)sequencing indicated that SUSP-4 markedly downregulated the level of Akkermansia and Alistipes.Metabolomics results confirmed that SUSP-4 obviously elevated thiamine levels compared with Model mice by adjusting thiamine metabolism,which was further confirmed by a targeted metabolism study.Fecal transplantation experiments showed that SUSP-4 exerted an anti-IBD effect by altering the intestinal flora in mice.A mechanistic study showed that SUSP-4 markedly inhibited macrophage activation by decreasing the levels of phospho-nuclear factor kappa-B(p-NF-κB)and cyclooxygenase-2(COX-2)and elevating NF-E2-related factor 2(Nrf2)levels compared with Model group.In conclusion,SUSP-4 affected thiamine metabolism by regulating Akker-mania and inhibited macrophage activation to adjust NF-κB/Nrf2/COX-2-mediated inflammation and oxidative stress against IBD.This is the first time that plant polysaccharides have been shown to affect thiamine metabolism against IBD,showing great potential for in-depth research and development applications.

Haochen Hui;Zhuoya Wang;Xuerong Zhao;Lina Xu;Lianhong Yin;Feifei Wang;Liping Qu;Jinyong Peng

Department of Pharmaceutical Analysis,College of Pharmacy,Dalian Medical University,Dalian,Liaoning,116044,ChinaInnovation Materials Research and Development Center,Botanee Research Institute,Yunnan Botanee Bio-technology Group Co.,Ltd.,Kunming,650106,ChinaDepartment of Pharmaceutical Analysis,College of Pharmacy,Dalian Medical University,Dalian,Liaoning,116044,China||Department of Traditional Chinese Medicine Pharmacology,School of Pharmacy,Anhui University of Chinese Medicine,Hefei,230012,China

Gut microbiotaInflammatory bowel diseasePolysaccharideSelaginella uncinata(Desv.)SpringThiamine metabolism

《药物分析学报(英文)》 2024 (002)

177-195 / 19

The authors gratefully acknowledge the funding from the Spring City Plan of the High-Level Talent Promotion and Training Project of Kunming,China(Grant No.:2022SCP008)and the Independent Research Fund of Yunnan Characteristic Plant Extraction Labora-tory,China(Grant No.:2022YKZY001).

10.1016/j.jpha.2023.08.003

评论