中国比较医学杂志2024，Vol.34Issue(4)：1-10,10.DOI:10.3969/j.issn.1671-7856.2024.04.001
CAST/EiJ小鼠外周血和脾中免疫细胞及亚群分析
Analysis of immune cells and their subsets in peripheral blood and spleen of CAST/EiJ mice
摘要
Abstract
Objective To explore the possible causes of CAST/EiJ mouse susceptibility to multiple pathogens,the immune cell phenotypes in the peripheral blood and spleen of CAST/EiJ mice were analyzed to clarify their composition.Methods Classical dendritic cells(cDCs),natural killer(NK)cells,B lymphocytes,T lymphocytes,and their subsets in the peripheral blood and spleen of CAST/EiJ mice and C57BL/6J mice were detected by flow cytometry using the cell surface markers CD3,CD4,CD8,CD11b,CD11c,CD19,CD27,CD49b,and TCRβ.Results There was no significant difference in the proportion of cDCs between CAST/EiJ and C57BL/6J mice,but the cDC1 cell subset population was smaller in CAST/EiJ.The proportions of NK cells(mainly mature NK cell subsets)and T lymphocytes(mainly CD8+T cells)were both lower in CAST/EiJ mice than C57BL/6J mice,while the proportion of B cells was higher in CAST/EiJ mice than C57BL/6J mice.Conclusions The proportions of NK and T lymphocytes in CAST/EiJ mice were lower than those in C57BL/6J mice.关键词
CAST/EiJ小鼠/C57BL/6J小鼠/经典型树突状细胞/自然杀伤细胞/B淋巴细胞/T淋巴细胞Key words
CAST/EiJ mice/C57BL/6J mice/classical dendritic cells/natural killer cells/B lymphocytes/T lymphocytes分类
医药卫生引用本文复制引用
刘琪,侯勇志,李娜,张京京,陆佳涵,丛喆,朱林,薛婧..CAST/EiJ小鼠外周血和脾中免疫细胞及亚群分析[J].中国比较医学杂志,2024,34(4):1-10,10.基金项目
国家自然科学基金(82241068) （82241068）
北京市自然科学基金(Z220018) （Z220018）
中国医学科学院医学与健康科技创新工程(2021-I2M-1-037). （2021-I2M-1-037）
