中国比较医学杂志2024，Vol.34Issue(4)：35-40,6.DOI:10.3969/j.issn.1671-7856.2024.04.005
FOXN1基因敲除兔嵌合体的建立
Establishment of chimeric rabbits with FOXN1 gene knockout
摘要
Abstract
Objective To establish F0 generation chimeric rabbits with FOXN1 gene knockout and explore method for the in vivo conservation of immunodeficient rabbits in a conventional housing environment.Methods Initially,CRISPR/Cas9 technology was employed to inject constructed sgRNA and Cas9 protein into a single cell from rabbit two-cell stage embryos to obtain chimeric embryos with FOXN1 gene editing.The embryos were subsequently transferred into surrogate does.Finally,the F0 generation offsprings were genotyped using PCR and Sanger sequencing,and their growth and development in a conventional housing environment were observed.Results The PCR and Sanger sequencing result confirmed the successful establishment of himeric rabbits with FOXN1 gene knockout.On observation,the chimeras exhibited normal growth and development in a conventional environment without any immunodeficient phenotypes.Conclusions This study established a preliminary chimeric rabbit model with FOXN1 gene knockout that grows and develops normally in standard laboratory environments.This lays the foundation for the further breeding of FOXN1 immunodeficient rabbits in the future.关键词
FOXN1/基因敲除兔/免疫缺陷/嵌合体Key words
FOXN1/gene knockout rabbit/immune deficiency/chimera分类
医药卫生引用本文复制引用
刘天平,黎桂玲,刘科,陈傍柱,王刚,顾为望..FOXN1基因敲除兔嵌合体的建立[J].中国比较医学杂志,2024,34(4):35-40,6.基金项目
国家自然科学基金(82101937) （82101937）
广东省科技计划项目(2019A030317012,2021B1212040016) （2019A030317012,2021B1212040016）
广东省医学科研基金项目(B2024069). （B2024069）
