轻工学报2024，Vol.39Issue(3)：9-20,12.DOI:10.12187/2024.03.002
1株甜菜糖蜜源高产胞外多糖明串珠菌的分离鉴定、发酵工艺优化及抗氧化活性研究
Isolation and identification,fermentation process optimization and antioxidant activity of a high exopolysaccharides-producing leuconostoc sp.from sugar beet molasses
摘要
Abstract
The objective of this study was to screen lactic acid bacteria strains with high exopolysaccharides production potential from sugar beet molasses.The taxonomic identification and growth characteristics of a lactic acid bacteria strain M1 with high exopolysaccharides-producing capacity isolated from sugar beet molasses were investigated.Furthermore,the exopolysaccharides fermentation parameters using sugar beet molasses as an alterna-tive carbon source to sucrose were optimized using a single-factor experiment and response surface methodology based on Box-Behnken design.Moreover,antioxidant activity of the harvested crude exopolysaccharides were eval-uated.The results showed that strain M1 was taxonomically classified as Leuconostoc suionicum.The growth profile of L.suionicum M1 indicated a lag phase of 2 hours,an optimal temperature of 35℃,a favorable pH value of 8.0,and a remarkable tolerance to sucrose concentrations up to 60％.The optimal medium for exopolysaccharides pro-duction consisted of 300 g/L sugar beet molasses,20.0 g/L yeast extract,15.0 g/L K2HPO4·3H2O,0.2 g/L MgSO4·7H2O,0.01 g/L NaCl,0.01 g/L CaCl2·2H2O,0.01 g/L MnSO4·4H2O,0.01 g/L FeSO4·7H2O.The maximum exopolysaccharides yield of 39.1 g/L was achieved under the following conditions:loading volume 200 mL/250 mL,inoculum size 5％,initial pH value 8.1,fermentation temperature of 38.5℃ and fermentation period 41.1 h.The exopolysaccharides produced by strain M1 exhibited significant antioxidant activities,with quenching capacities of 70.25％for DPPH radicals,47.16％for·OH radicals,35.92％for ABTS+radicals and 0.42 for Fe3+total reducing force at a concentration of 6.0 mg/mL.Strain M1 was capable of producing exopolysac-charides with antioxidant activity using sugar beet molasses,and could be used as a promising candidate for the bioconversion of sugar beet molasses into functional exopolysaccharides products.关键词
苏奥古姆明串珠菌/甜菜糖蜜/胞外多糖/发酵工艺/抗氧化活性Key words
Leuconostoc suionicum/sugar beet molasses/exopolysaccharide/fermentation process/antioxidant activity分类
轻工纺织引用本文复制引用
张菡,陈海军,龙晓宇,赵抒娜,魏学军,魏国坤,申光辉..1株甜菜糖蜜源高产胞外多糖明串珠菌的分离鉴定、发酵工艺优化及抗氧化活性研究[J].轻工学报,2024,39(3):9-20,12.基金项目
2022年新疆维吾尔自治区专利转化项目(20220023) （20220023）
昌吉回族自治州科技计划项目(2022X01) （2022X01）
中粮糖业控股股份有限公司-四川农业大学校企合作项目(060-2222319002) （060-2222319002）
2021年度新疆维吾尔自治区昌吉回族自治州博峰精英计划柔性领军人才项目(2022015) （2022015）
