耐高温异养硝化-好氧反硝化菌的分离鉴定及其脱氮特性研究OA

Through the study of 16S rDNA and physiological and biochemical characteristics of various strains,a heterotrophic nitrification and aerobic denitrification bacteria(HN-AD)strain with good heat resistance was found.The strain was inoculated into a nitrate nitrogen culture medium simulating sewage environment to study its denitrification efficiency and nitrate nitrogen removal rate under insufficient and sufficient carbon sources.The results showed that HN-AD strain that could make the pH of wastewater more suitable for its own denitrification was screened in hot springs,and the identified HN-AD strain was Pseudomonas stutzeri.The optimal pH for denitrification of this strain in a simula-ted wastewater environment was 8.0,with sufficient carbon source,its nitrate nitrogen removal rate could reach over 93%.The strain not only had a relatively efficient denitrification efficiency and a high nitrate nitrogen removal rate,but also had the ability to self regulate the environ-mental pH,which could be adjusted to the optimal pH for nitrogen degradation under sufficient carbon sources.