安徽农业科学2024，Vol.52Issue(12)：7-12,6.DOI:10.3969/j.issn.0517-6611.2024.12.002
耐高温异养硝化-好氧反硝化菌的分离鉴定及其脱氮特性研究
Isolation,Identification and Denitrification Characteristics of High-temperature Tolerant Heterotrophic Nitrification and Aerobic Denitrification
摘要
Abstract
Through the study of 16S rDNA and physiological and biochemical characteristics of various strains,a heterotrophic nitrification and aerobic denitrification bacteria(HN-AD)strain with good heat resistance was found.The strain was inoculated into a nitrate nitrogen culture medium simulating sewage environment to study its denitrification efficiency and nitrate nitrogen removal rate under insufficient and sufficient carbon sources.The results showed that HN-AD strain that could make the pH of wastewater more suitable for its own denitrification was screened in hot springs,and the identified HN-AD strain was Pseudomonas stutzeri.The optimal pH for denitrification of this strain in a simula-ted wastewater environment was 8.0,with sufficient carbon source,its nitrate nitrogen removal rate could reach over 93%.The strain not only had a relatively efficient denitrification efficiency and a high nitrate nitrogen removal rate,but also had the ability to self regulate the environ-mental pH,which could be adjusted to the optimal pH for nitrogen degradation under sufficient carbon sources.关键词
污水处理/耐高温异养硝化-好氧反硝化菌/分离鉴定/脱氮特性Key words
Sewage treatment/High temperature tolerant heterotrophic nitrification and aerobic denitrification bacteria(HN-AD)/Isolation and identification/Denitrification characteristics分类
资源环境引用本文复制引用
毕思远,罗傲,廖建珍,房志家,羊世成,李新红,赵聪..耐高温异养硝化-好氧反硝化菌的分离鉴定及其脱氮特性研究[J].安徽农业科学,2024,52(12):7-12,6.基金项目
广西博士后专项经费资助项目(202320) （202320）
湛江市科技计划项目(2022A01014) （2022A01014）
国家自然科学基金青年科学基金项目(62205221). （62205221）
