农业生物技术学报2024，Vol.32Issue(7)：1504-1517,14.DOI:10.3969/j.issn.1674-7968.2024.07.004
豌豆NAC基因家族鉴定及其在干旱胁迫下的响应分析
Identification of NAC Gene Family and Its Response Analysis Under Drought Stress in Pisum sativum
摘要
Abstract
The NAC is one of the plant specific transcription factor and plays a key role in plant growth,development and stress responses.In this study,84 NAC genes were identified from Pisum sativum genome database by sequence alignment using the NAC family genes of Arabidopsis thaliana and Glycine max.They were distributed unevenly on 7 chromosomes and divided into 13 subfamilies.All subfamily members had highly homologous sequences at the N-terminal region and highly divergent sequences at the C-terminal region.Members of the same subfamily had similar types and quantities of conserved motifs,and contain specific conserved motifs.Most NAC genes contained 3 exons.There were 29 and 50 NAC genes in the pea genome,which were collinear with the NAC genes in the Arabidopsis and soybean genome,respectively.Seventy-nine genes contained at least one abiotic stress related element such as drought responsive element and low-temperature responsive element.The drought stress expression profile showed that 60 PsNAC genes had obvious response to drought stress.75.0%and 81.7%of the 60 PsNAC showed significant response in the early stage and late stage of drought stress,respectively,indicating that PsNAC gene played an important role in different stages of drought in peas.Among them,five PsNAC genes had higher response level in the shoot or root within 48 h of drought stress.This study analyzed the information of PsNAC gene family members which could provide a reference for further research on identification of stress resistance related genes.关键词
豌豆/NAC基因家族/生物信息学分析/干旱胁迫Key words
Pea/NAC gene family/Bioinformatics analysis/Drought stress分类
农业科技引用本文复制引用
刘飞,杨亲康,何德钏,邢宝龙,李梦蛟..豌豆NAC基因家族鉴定及其在干旱胁迫下的响应分析[J].农业生物技术学报,2024,32(7):1504-1517,14.基金项目
国家食用豆产业技术体系大同综合试验站(CARS-08-Z5) （CARS-08-Z5）
山西农业大学生物育种工程项目(YZGC078) （YZGC078）
