豌豆NAC基因家族鉴定及其在干旱胁迫下的响应分析OA北大核心CSTPCD

The NAC is one of the plant specific transcription factor and plays a key role in plant growth,development and stress responses.In this study,84 NAC genes were identified from Pisum sativum genome database by sequence alignment using the NAC family genes of Arabidopsis thaliana and Glycine max.They were distributed unevenly on 7 chromosomes and divided into 13 subfamilies.All subfamily members had highly homologous sequences at the N-terminal region and highly divergent sequences at the C-terminal region.Members of the same subfamily had similar types and quantities of conserved motifs,and contain specific conserved motifs.Most NAC genes contained 3 exons.There were 29 and 50 NAC genes in the pea genome,which were collinear with the NAC genes in the Arabidopsis and soybean genome,respectively.Seventy-nine genes contained at least one abiotic stress related element such as drought responsive element and low-temperature responsive element.The drought stress expression profile showed that 60 PsNAC genes had obvious response to drought stress.75.0%and 81.7%of the 60 PsNAC showed significant response in the early stage and late stage of drought stress,respectively,indicating that PsNAC gene played an important role in different stages of drought in peas.Among them,five PsNAC genes had higher response level in the shoot or root within 48 h of drought stress.This study analyzed the information of PsNAC gene family members which could provide a reference for further research on identification of stress resistance related genes.