农业生物技术学报2024，Vol.32Issue(7)：1518-1532,15.DOI:10.3969/j.issn.1674-7968.2024.07.005
姜OSCA基因家族的鉴定及表达分析
Identification and Expression Analysis of OSCA Gene Family in Ginger(Zingiber officinale)
摘要
Abstract
Hyperosmolarity gate calcium-permeable channels(OSCA)is a mechanosensitive porous ion channel,which can regulate Ca2+concentration and water transport rate in plant cells under osmotic stress.It plays an important role in hypertonic stress in plants.In order to understand the structural characteristics and expression characteristics of OSCA gene family in ginger,this study identified and analyzed the OSCA gene family in ginger(Zingiber officinale).The results showed that 38 members of OSCA gene family were identified from ginger genome,which were located on 14 chromosomes respectively.The prediction of subcellular localization shows that the members of OSCA family in ginger were mainly located in plasma membrane.The analysis of cis-acting elements of promoters showed that 38 ZoOSCAs contained various response elements to adversity and hormone,such as methyl jasmonate,abscisic acid and drought.The transcriptome of salt stress and fluorescence quantitative results of drought treatment showed that the expression levels of 18,23 family members in leaves and rhizomes were up-regulated under salt stress,and 11 out of 14 family members were up-regulated under drought treatment,and the expression levels of ZoOSCAs in different tissues were different.This study provides basic data for revealing the function and mechanism of ZoOSCAs.关键词
姜/钙通透性阳离子通道蛋白(OSCA)/基因家族/鉴定/表达分析Key words
Ginger/Hyperosmolarity-gate calcium-permeable channels(OSCA)/Gene family/Identification/Expression analysis分类
农业科技引用本文复制引用
龙茶,吕金丽,夏应书,李伟,汪淘,花河兰,吴媛,苏世贤,韦金江,江苏燕,牟海林,祝洪江..姜OSCA基因家族的鉴定及表达分析[J].农业生物技术学报,2024,32(7):1518-1532,15.基金项目
贵州省高等学校设施蔬菜工程研究中心平台建设项目(黔教技[2022]040号),贵州省农业农村厅生姜小分队(第一批农村产业革命)蔬菜产业发展项目(黔财农[2020]98号) （黔教技[2022]040号）
农业农村部/贵州省农业农村厅镇宁县2022年贵州高原夏秋蔬菜优势特色产业集群项目(黔财农[2022]89号) （黔财农[2022]89号）
