西蒙得木HSP20基因家族的进化、表达和功能分析OA北大核心CSTPCD

[Objective]Clarifying the structure and function of members of the jojoba HSP20 family(ScHSP20)will provide support for subsequent study.[Method]A systematic analysis was conducted on the ScHSP20 family members in jojoba from structural,phylogenetic,and expression perspectives,and functional analysis of ScHSP20-17 was performed.[Result]The genome of jojoba encompassed 35 HSP20 gene loci,with 8 genes involved in tandem duplications and 5 in segmental duplications.Phylogenetic analysis showed that 35 ScHSP20 clusters into 12 subfamilies,with the highest number of nuclear cytoplasmic subfamilies.Promoter analysis revealed that the majority of ScHSP20 family members possessed cis-acting elements in their promoter regions associated with the responses to hormone and abiotic stress.Transcriptome analysis indicated that the expression levels of some ScHSP20 genes,such as ScHSP20-35 and ScHSP20-17,underwent significant changes during flower and seed development,and genes like ScHSP20-17 and ScHSP20-28 were induced by high temperatures.Subcellular localization analysis showed that the protein encoded by ScHSP20-17 was located in the nucleus,and the overexpression of ScHSP20-17 in Escherichia coli,Saccharomyces cerevisiae,and Nicotiana benthamiana markedly improved their tolerance to high temperature stress.[Conclusion]Some members of the ScHSP20 family are involved in the reproductive development and response of jojoba to abiotic stress.