生物技术通报2024，Vol.40Issue(6)：190-202,13.DOI:10.13560/j.cnki.biotech.bull.1985.2024-0012
西蒙得木HSP20基因家族的进化、表达和功能分析
Evolution,Expression,and Functional Analysis of the HSP20 Gene Family from Simmondisa chinensis
摘要
Abstract
[Objective]Clarifying the structure and function of members of the jojoba HSP20 family(ScHSP20)will provide support for subsequent study.[Method]A systematic analysis was conducted on the ScHSP20 family members in jojoba from structural,phylogenetic,and expression perspectives,and functional analysis of ScHSP20-17 was performed.[Result]The genome of jojoba encompassed 35 HSP20 gene loci,with 8 genes involved in tandem duplications and 5 in segmental duplications.Phylogenetic analysis showed that 35 ScHSP20 clusters into 12 subfamilies,with the highest number of nuclear cytoplasmic subfamilies.Promoter analysis revealed that the majority of ScHSP20 family members possessed cis-acting elements in their promoter regions associated with the responses to hormone and abiotic stress.Transcriptome analysis indicated that the expression levels of some ScHSP20 genes,such as ScHSP20-35 and ScHSP20-17,underwent significant changes during flower and seed development,and genes like ScHSP20-17 and ScHSP20-28 were induced by high temperatures.Subcellular localization analysis showed that the protein encoded by ScHSP20-17 was located in the nucleus,and the overexpression of ScHSP20-17 in Escherichia coli,Saccharomyces cerevisiae,and Nicotiana benthamiana markedly improved their tolerance to high temperature stress.[Conclusion]Some members of the ScHSP20 family are involved in the reproductive development and response of jojoba to abiotic stress.关键词
西蒙得木/HSP20/亚细胞定位/表达分析/高温胁迫Key words
jojoba/HSP20/subcellular localization/expression analysis/heat stress引用本文复制引用
李博静,郑腊梅,吴乌云,高飞,周宜君..西蒙得木HSP20基因家族的进化、表达和功能分析[J].生物技术通报,2024,40(6):190-202,13.基金项目
国家自然科学基金项目(31770363,31670335),"质谱成像与代谢组学国家民委重点实验室"自主课题(KLMSIM202305),中央民族大学研究生自主科研项目(SZKY2023081) （31770363,31670335）
