丝羽乌骨鸡SLC38A11基因分型及其与皮肤乌色度关联分析OA北大核心CSTPCD

To further verify the role of SLC38A11 in melanin deposition in black bone chickens,this study genotyped the SNPs loci of the SLC38A11 gene based on time-of-flight mass spectrometry analysis technology.Hapoview 4.1 was used to analyze the linkage disequilibrium of all SNP sites,and detected whether the SNP sites were in an H-W equilibrium state,and analyzed the correlation between genotype and skin brightness L*value,and screened SNP markers that were significantly correlated with skin blackness.The results were as follows:1)The mass spectrometry detection rate of four polymorphic loci in the SLC38A11 gene was 100％,and each locus had three genotypes.The SNP1 of SLC38A11 gene was a missense mutation,the SNP2,SNP3 and SNP4 were all synonymous mutations.2)Chi-squared test showed that SNP1 deviated from Hardy-Weinberg equilibrium balance(P＜0.05)and the other three SNPs were in Hardy-Weinberg equilibrium balance(P＞0.05).The genetic diversity of SNP1 locus was lower,and the other three SNPs loci were moderately polymorphic.3)Single marker association analysis showed that the breast skin brightness L*value of the TC genotype at the SNP1 locus was significantly lower than that of the CC and TT genotypes(P＜0.05).The breast skin brightness L*value of the AA genotype at the SNP2 locus was significantly lower than that of the GG and GA genotypes(P＜0.05).The breast skin brightness L*value of the TT genotype at the SNP3 locus was significantly lower than that of the CC and CT genotypes(P＜0.05).4)The Linkage disequilibrium analysis of four SNPs showed that the strong linkage among SNP2,SNP3 and SNP4 produced three Haplotypes.Association analysis found that the breast skin color brightness L*value of H3H3 Haplotype(AATTAA genotype)was significantly lower than that of Haplotype H2H3(GACTAG)and H2H1(GGCCAA).After the combination of SNP1-SNP4 genotypes,the breast skin color brightness L*value of TCAATTAA was significantly lower than that of CCGGCCAA,TTGACTAG and CCGACTAG.the TCAATTAA genotype had a lower L*value for breast skin brightness and a higher blackness.In summary,the SLC38A11 gene is closely related to the breast skin blackness,and the TCAATTAA genotype can be used as a candidate molecular marker for studying the breast skin blackness in Silky fowls.It will provide a theoretical reference for using molecular marker assisted breeding to accelerate the cultivation of new Silky fowls with higher blackness.