中国农业大学学报2024，Vol.29Issue(7)：1-11,11.DOI:10.11841/j.issn.1007-4333.2024.07.01
丝羽乌骨鸡SLC38A11基因分型及其与皮肤乌色度关联分析
Genotyping of SLC38A11 gene in Silky fowls and its association with skin blackness
摘要
Abstract
To further verify the role of SLC38A11 in melanin deposition in black bone chickens,this study genotyped the SNPs loci of the SLC38A11 gene based on time-of-flight mass spectrometry analysis technology.Hapoview 4.1 was used to analyze the linkage disequilibrium of all SNP sites,and detected whether the SNP sites were in an H-W equilibrium state,and analyzed the correlation between genotype and skin brightness L*value,and screened SNP markers that were significantly correlated with skin blackness.The results were as follows:1)The mass spectrometry detection rate of four polymorphic loci in the SLC38A11 gene was 100％,and each locus had three genotypes.The SNP1 of SLC38A11 gene was a missense mutation,the SNP2,SNP3 and SNP4 were all synonymous mutations.2)Chi-squared test showed that SNP1 deviated from Hardy-Weinberg equilibrium balance(P＜0.05)and the other three SNPs were in Hardy-Weinberg equilibrium balance(P＞0.05).The genetic diversity of SNP1 locus was lower,and the other three SNPs loci were moderately polymorphic.3)Single marker association analysis showed that the breast skin brightness L*value of the TC genotype at the SNP1 locus was significantly lower than that of the CC and TT genotypes(P＜0.05).The breast skin brightness L*value of the AA genotype at the SNP2 locus was significantly lower than that of the GG and GA genotypes(P＜0.05).The breast skin brightness L*value of the TT genotype at the SNP3 locus was significantly lower than that of the CC and CT genotypes(P＜0.05).4)The Linkage disequilibrium analysis of four SNPs showed that the strong linkage among SNP2,SNP3 and SNP4 produced three Haplotypes.Association analysis found that the breast skin color brightness L*value of H3H3 Haplotype(AATTAA genotype)was significantly lower than that of Haplotype H2H3(GACTAG)and H2H1(GGCCAA).After the combination of SNP1-SNP4 genotypes,the breast skin color brightness L*value of TCAATTAA was significantly lower than that of CCGGCCAA,TTGACTAG and CCGACTAG.the TCAATTAA genotype had a lower L*value for breast skin brightness and a higher blackness.In summary,the SLC38A11 gene is closely related to the breast skin blackness,and the TCAATTAA genotype can be used as a candidate molecular marker for studying the breast skin blackness in Silky fowls.It will provide a theoretical reference for using molecular marker assisted breeding to accelerate the cultivation of new Silky fowls with higher blackness.关键词
乌骨鸡/SLC38A11/乌色度/SNP位点/连锁不平衡Key words
black-bone chickens/SLC38A11/blackness/SNP sites/linkage disequilibrium分类
农业科技引用本文复制引用
屠云洁,郑国庆,章明,巨晓军,姬改革,刘一帆,单艳菊,邹剑敏,束婧婷,赵伟东..丝羽乌骨鸡SLC38A11基因分型及其与皮肤乌色度关联分析[J].中国农业大学学报,2024,29(7):1-11,11.基金项目
江苏省农业自主创新基金项目(CX(21)2011-1) （CX(21）
江苏省种业振兴揭榜挂帅项目(JBGS[2021]107) （JBGS[2021]107）
现代农业产业技术体系建设专项资金(CARS-41) （CARS-41）
扬州市现代农业项目(YZ2021029) （YZ2021029）
江苏省现代农业产业技术体系(JATS[2023J396]) （JATS[2023J396]）
