植物营养与肥料学报2024，Vol.30Issue(5)：934-947,14.DOI:10.11674/zwyf.2023555
硝普钠添加对碱胁迫下萌发期水稻碳氮代谢的影响
Effect of sodium nitroprusside addition on carbon and nitrogen metabolism of rice during germination under alkali stress
摘要
Abstract
[Objectives]The application of exogenous sodium nitroprusside(SNP)can effectively alleviate alkali stress-induced damage in rice.We observed the effect differences of exogenous SNP on the carbon and nitrogen metabolism of rice cultivars when subjecting to alkali stress at germination satge.[Methods]Alkali sensitive rice cultivar Zhonghua 11(ZH11)and alkali resistant cultivar Ninggeng 52(NG52)was chosed as test materials in a pot experiment.Firstly,rice seeds were soaked into alkali stress solutions(containing 20 mmol/L CO32-and HCO3-,pH 10.50)containing SNP 10,30,50,70,100 μmol/L,respectively.SNP 30 μmol/L treatment was recorded the highest germination index,so was used as the SNP concentrations for the following experiment.In the following experiment,normal nutrient solution control(CK),normal solution containing SNP 30 μmol/L(SNP),alkali stress solution(AS),and alkali stress solution containing NSP 30 μmol/L(AS+SNP).The rice seeds were watered with the treatment solution,and the carbon and nitrogen metabolism of rice seedlings were measured.[Results]AS+SNP treatment were recorded significantly lower sucrose content than AS treatment in both cultivar ZH11 and NG52.The sucrose contents of NG52 were significantly lower than that of ZH11,however,the expression of genes involved in sucrose synthesis(OsNIN1)and glucose synthesis(OsSPS1 and OsSPS11)in NG52 were significantly higher than that in ZH11.Compared with AS,the AS+SNP treatment markedly increased the levels of malic acid,citric acid,nitric oxide(NO),and proline content,as well as the activities of nitrate reductase(NR)and S-nitroso glutathione reductase(GSNOR)and expression of OsNR2 and OsGSNOR1,decreased the levels of NO3-and glutathione(oxidized glutathione,GSSG)in both cultivars.Furthermore,the contents of malic acid,citric acid,NO,proline,GSSG,together with GSNOR activity and the expression levels of OsNR1.2 and OsGSNOR1 were significantly higher in NG52 compared with ZH11.Positive correlations(P<0.05)were existed between genes involved in glucose and sucrose synthesis(OsNIN1)with malic acid,citric acid,proline,and NO.The principal component analysis indicated that glucose,fructose,NO,GSNO,and GSSG were key factors affecting the coordination of carbon and nitrogen metabolism.[Conclusions]Treating rice seeds with 30 μmol/L SNP solution is effective in increasing sucrose breakdown,organic acid accumulations,NR and GSNOR activities,organic nitrogen levels,and stimulating the transformation of carbon metabolism to nitrogen metabolism,thereby maintaining the normal carbon and nitrogen metabolism of rice under alkali stress during germination.关键词
水稻/萌发期/碱胁迫/硝普纳/碳氮代谢Key words
rice/germination/alkali stress/sodium nitroprusside/carbon and nitrogen metabolism引用本文复制引用
闵炜芳,方晶莹,石亚飞,摆小蓉,舍杨梦斐,田浩天,罗成科..硝普钠添加对碱胁迫下萌发期水稻碳氮代谢的影响[J].植物营养与肥料学报,2024,30(5):934-947,14.基金项目
宁夏自然科学基金项目(2023AAC03144) （2023AAC03144）
宁夏科技创新团队项目(2022BSB03109) （2022BSB03109）
宁夏大学大学生创新创业训练计划项目(S202310749101). （S202310749101）
