果树学报2024，Vol.41Issue(6)：1054-1063,10.DOI:10.13925/j.cnki.gsxb.20230561
桃果皮叶绿素降解规律与相关基因表达分析
Analysis of chlorophyll degradation and related gene expression in peach peel
摘要
Abstract
[Objective]Peach(Prunus persica),is one of the most important deciduous fruit trees in the world.The visual appeal of peach fruits,particularly their color,is a critical factor influencing consum-er purchasing decisions.Chlorophyll content is important in determining the skin color and overall ap-pearance of peaches.The precise role of chlorophyll degradation genes within the peach genome needs to fully understand,and their potential effects on chlorophyll breakdown in the peach peel and fruit rip-ening remain unclear.This study aimed to lay preliminary groundwork for understanding the ripening period and the underlying color differences in peach fruits by examining the color changes in the peach peels during the process of chlorophyll degradation,and the expression patterns of the genes related to the chlorophyll degradation.[Methods]Four peach varieties including Chunmei and 2-50 were chosen for this investigation.The color variations in the peach fruits prior to ripening were quantified using a colorimeter,and chlorophyll content was measured with a UV spectrophotometer.Although the primary chlorophyll degradation pathway has been identified,there are only limited researches on gene expres-sion in the fruits.To fill this gap,quantitative transcription analysis was conducted,and the coding se-quences(CDS)of relevant genes were sourced from genomic databases.The expression of the chloro-phyll degradation-associated genes was quantified using real-time quantitative polymerase chain reac-tion(qRT-PCR),and the correlation between the chlorophyll degradation and the gene expression was established through statistical analysis.[Results]The L*value of the peach fruit color difference index increased gradually from 30 to 16 days before ripening and then decreased as the fruit matured.All the varieties exhibited an S-type a*value trend,with a*experiencing a sharp rise from 23 to 12 days prior to ripening.During the 12 days before ripening,the green hue of the peach fruit faded,and changed to red and showed a continuous increase in the a*value.The a*value shifted from negative to positive or near zero from 12 to 8 days before ripening,signifying the pivotal period for color change.The b*val-ue initially increased from 30 to 16 days before ripening and then decreased,and the yellow-fleshed va-rieties showed higher values than the white-fleshed ones.The chlorophyll content significantly de-creased in all the varieties as the fruit developed,and a steep decline was observed in 30 and 16 days be-fore maturity.From 12 to 8 days before ripening,the chlorophyll levels of the four varieties fell below 8 pg·g-1,indicating visible coloring,though ripening was still not completed.The high expression levels of the PpCLH2 and the PpSGRL were detected 30 days before maturity when the fruit was entirely green.The expression levels decreased as the fruit ripened,while the PpCLH1 expression peaked dur-ing the critical period of incomplete coloring and color transformation,suggesting that it would play a pivotal role in the chlorophyll degradation.The PpPPH,PpPAO and PpRCCR genes showed increased expression from 8 to 0 days before ripening,while the PpSGR was significantly overexpressed as the fruit matured,indicating that it would play a primary role in the chlorophyll degradation.The gene clus-ter analysis and correlation studies further highlighted the significant and negative correlation between the PpSGR expression and the chlorophyll content,underscoring its key function during peach fruit rip-ening.[Conclusion]This research would provide novel insights into the expression of the genes related to the chlorophyll degradation and their relationship with the color change and the chlorophyll content in the peach peels.We identified that the period approximately 12 to 8 days before ripening would be critical for the color transition in the peach peels,and the PpCLH1 and PpSGR would be the key genes in the pre-ripening chlorophyll degradation process.These findings would not only enhance our under-standing of the chlorophyll degradation throughout peach fruit development but also offer a valuable ref-erence for future studies on the molecular mechanisms of the chlorophyll degradation in peaches.关键词
桃/果皮/叶绿素降解/PpCLH1基因/PpSGR基因Key words
Peach/Peel/Chlorophyll degradation/PpCLH1 gene/PpSGR gene分类
农业科技引用本文复制引用
刘鑫,张晓煜,孟君仁,段文宜,孙世航,潘磊,曾文芳,王志强,牛良..桃果皮叶绿素降解规律与相关基因表达分析[J].果树学报,2024,41(6):1054-1063,10.基金项目
国家桃产业技术体系(CARS-30) （CARS-30）
中国农业科学院科技创新工程专项经费项目(CAAS-ASTIP-2023-ZFRI) （CAAS-ASTIP-2023-ZFRI）
河南省重大公益科技专项(201300110500) （201300110500）
