河南农业大学学报2024，Vol.58Issue(3)：412-423,12.DOI:10.16445/j.cnki.1000-2340.20240301.002
桃基因PpNAC的鉴定及其在不同发育时期的表达分析
Identification and expression analysis of PpNAC genes during different development stages of peach fruit
摘要
Abstract
[Objective]The study aims to identify the PpNAC genes affecting peach fruit ripening and analyze its expression changes in different developmental stages of peach fruit for laying a foundation to understand the molecular mechanism of peach fruit ripening.[Method]Transcriptome analysis of differ-ent tissues were used to identify the PpNAC genes highly expressed in peach fruit from 115 PpNAC gene family members.Further bioinformatics analysis,protein interaction analysis and expression anal-ysis were analyzed.[Result]A number of 16 PpNAC genes highly expressed in peach fruit were identi-fied,and distributed on different 8 chromosomes.The protein molecular weight and isoelectric point were different.The coding region of gene contained the NAM conserved domain and motif 1-6,inclu-ding 3-8 exons and 2-7 introns.The most numerous cis-regulatory elements in promoters were involved in abscisic acid and jasmonic acid.In addition,four PpNAC proteins interacting with the PpNAC1 were obtained by interaction screening with a reported NAC member regulating peach fruit ripening.Further expression analysis showed that the expression levels of these four PpNAC genes reached the highest at the beginning and late stages of fruit ripening.[Conclusion]The PpNAC genes may partici-pate in the regulation of peach fruit ripening.关键词
桃/NAC基因/果实成熟/表达分析Key words
peach/NAC gene/fruit ripening/expression analysis分类
农业科技引用本文复制引用
王晓菲,张郎郎,高利盈,刘宁,程钧,王伟,谭彬,郑先波,叶霞,冯建灿..桃基因PpNAC的鉴定及其在不同发育时期的表达分析[J].河南农业大学学报,2024,58(3):412-423,12.基金项目
国家自然科学基金青年项目(32002014) （32002014）
国家重点研发计划项目(2019YFD1000104) （2019YFD1000104）
