河南农业大学学报2024，Vol.58Issue(3)：435-443,9.DOI:10.16445/j.cnki.1000-2340.20230918.001
携带blaCTX-M禽大肠杆菌的多重耐药特征和体外致病性研究
Multi-drug resistance characteristics and in vitro pathogenicity in blaCTX-M-carrying avian Escherichia coli
摘要
Abstract
[Objective]To further understand the molecular epidemic characteristics of bla CTX-M-carrying avian Escherichia coli.[Method]The multiple drug resistance profile,phylogenetic grouping,and virulence genes of 76 strains of blaCTX-M-carrying avian Escherichia coli were detected,and the correlation between virulence genes and drug resistance characteristics,as well as phylogenetic groups,was analyzed.[Result]Antimicrobial susceptibility test results showed that blaCTX-M-carrying avian Escherichia coli had the highest antibiotic resistance rate to florfenicol,reaching 85.53％(65/76),followed by enrofloxacin 48.68％(37/76),and the most common multidrug resistance spectrum was florfenicol+enrofloxacin+acetaquine,reaching 10.53％(8/76).Through multiple PCR for phylogenetic groups,it was found that the main subgroups were group C and group B1,and the group C has the highest detection rate of 53.95％(41/76),followed by group B1,with 30.26％(23/76).The detection rates of group E,group A,group D,and group F were 9.21％(7/76),2.63％(2/76),2.63％(2/76),and 1.32％(1/76),respectively.None of strains belonged to group B2 and branch I.The results of virulence genes testing showed that at least 4 virulence factors were carried in 76 strains of bla CTX-M-carrying avian Escherichia coli,and 11 strains carried at least 11 virulence genes at the same time.Among them,13 strains simultaneously carried five marker virulence genes(iroN,iutA,hly,OmpT and iss)of avian pathogenic Escherichia coli(APEC),with a detection rate of 17.11％,while 11 strains had marker genes of ColV plasmid(cva/cvi,iroN,iucD/iutA,etsAC,OmpT/hlyF,and sitA),with a detection rate of 14.47％,and 28 strains harbored high pathogenitility island(HPI)marker genes irp-2 and fyuA,with a detection rate of 36.82％.[Conclusion]The blaCTX-M-carrying avian Escherichia coli is mostly multidrug resistant,and the multidrug resistance of the bacteria is more likely to carry ColV virulence plasmid.The multidrug resistance of blaCTXM-carrying avian Escherichia coli is positive correlation with its pathogenicity.关键词
禽大肠杆菌/多重耐药/系统进化分群/毒力基因/ColV质粒Key words
avian Escherichia coli/multidrug resistance/phylogenetic group/virulence gene/ColV plasmid分类
农业科技引用本文复制引用
崔俊岭,梁玉蕾,刘新新,楚浩然,陈秋如,董焱斌,贺丹丹,吴华,苑丽..携带blaCTX-M禽大肠杆菌的多重耐药特征和体外致病性研究[J].河南农业大学学报,2024,58(3):435-443,9.基金项目
河南省高校科技创新团队支持计划项目(23IRSTHN021) （23IRSTHN021）
河南省自然科学基金重点项目(232300421111) （232300421111）
