激光生物学报2024，Vol.33Issue(3)：236-242,7.DOI:10.3969/j.issn.1007-7146.2024.03.006
人脐带间充质干细胞治疗妊娠期糖尿病小鼠的机制研究
Mechanism Study of Human Umbilical Cord Mesenchymal Stem Cells in the Treatment of Mice with Gestational Diabetes
摘要
Abstract
s:This study aimed to investigate the the effectiveness and mechanism of human umbilical cord mesenchymal stem cells(hUCMSCs)in mice with gestational diabetes mellitus(GDM)model,and to study a new method for the treatment of GDM.hUCMSCs were obtained by tissue block culture.Female mice were raised with intraperitoneal injection of streptozoto-cin(STZ)solution to induce type 2 diabetes after being fed with D12451 high-fat.Then,mice with type 2 diabetes mellitus were kept in cages overnight,both male and female.Sperm were examined the next morning using pubic plugs and microscopic sperm,which was recorded as the first day of gestation,and fasting blood glucose was measured on the fourth day of gestation.The animals with fasting blood glucose higher than of 11.2 mmol/L and polydipsia,polyphagia,and polyuria were designated as GDM model mice.After injecting 5×105 hUCMSCs into the tail vein to treat GDM model mice,the effects of hUCMSCs on GDM mice were analyzed by detecting changes in blood glucose levels,serum inflammatory factors,macrophages,pathologi-cal sections of islet cells,and other indicators.Blood glucose levels of GDM mice treated with hUCMSCs were significantly reduced(0.001<P<0.01).Immunohistochemical results showed that the ratio of islet-β-cells to α-cells increased significantly in the mice after treatment with hUCMSCs(0.001<P<0.01).The results of inflammatory factor detection showed that the ex-pression of pro-inflammatory factor IL-1β was reduced in the treatment group compared with the control group(P<0.05).In vitro cell experiments showed an increase in the expression of M1 macrophages co-cultured with hUCMSCs.It indicated that hUCMSCs can reduce the blood glucose level of GDM mice to a certain extent and effectively affect their immunomodulatory effect,which is beneficial to the treatment of GDM in mice.关键词
人脐带间充质干细胞/妊娠期糖尿病/炎症因子/糖代谢/模型小鼠Key words
human umbilical cord mesenchymal stem cells/gestational diabetes mellitus/inflammatory factors/sugar metab-olism/model mice分类
医药卫生引用本文复制引用
杨静,刘芬,李毅,吴东波,邓曼丹,阳成英,尹晨,胡翔..人脐带间充质干细胞治疗妊娠期糖尿病小鼠的机制研究[J].激光生物学报,2024,33(3):236-242,7.基金项目
湖南省自然科学基金项目(2021JJ70058) （2021JJ70058）
国家自然科学基金面上项目(81972642). （81972642）
