肉类研究2024，Vol.38Issue(4)：17-22,6.DOI:10.7506/rlyj1001-8123-20240320-059
酶解提取肉鸽血液中血红素铁的工艺优化
Optimization of the Enzymatic Extraction of Heme Iron from Pigeon Blood
摘要
Abstract
In this research,heme iron from meat-type pigeon blood was extracted by enzymatic hydrolysis using papain,neutral protease,alkaline protease,flavourzyme or protamex.Based on heme iron content,papain and protamex were selected for dual-enzymatic hydrolysis.Employing one-factor-at-a-time(OFAT)method and orthogonal array design(OAD)with heme iron content and hydrolysis degree as evaluation indicators,the optimum extraction conditions were determined as follows:papain-to-protamex ratio 2:1,pH 7.0,temperature 50℃,blood-to-water ratio of 1:2,enzyme dosage 8 000 U/g,and hydrolysis time 3 h.Under these conditions,the extraction yield of heme iron was 95.43 μg/mL,which was significantly increased compared with that obtained using single enzymes.关键词
肉鸽血液/血红素铁/酶法提取/工艺优化Key words
pigeon blood/heme iron/enzymatic extraction/process optimization分类
轻工纺织引用本文复制引用
张远红,张纬嘉,李滢,陈丽婷,陈伟波,曾晓房..酶解提取肉鸽血液中血红素铁的工艺优化[J].肉类研究,2024,38(4):17-22,6.基金项目
梅州市科技计划项目(2023A0304001) （2023A0304001）
