农业环境科学学报2024，Vol.43Issue(6)：1350-1359,10.DOI:10.11654/jaes.2023-0660
稻鳝种养模式对土壤氨氧化微生物群落多样性和结构的影响
Effects of the rice-eel breeding model on the diversity and structure of soil ammonia-oxidizing microbial communities
摘要
Abstract
Research on the composition,structure,and diversity changes of soil ammonia-oxidizing microbial communities under the rice-eel breeding model is needed to gain in-depth insights into the impact on key microbes involved in soil nitrogen cycling.In this study,Illumina MiSeq high-throughput sequencing was systematically employed to analyze the community structure and diversity of ammonia-oxidizing microbes[ammonia oxidizing archaea(AOA),ammonia oxidizing bacteria(AOB)and complete ammonia oxidizer(Comammox)]in the rice-eel breeding model.Two treatments were established:conventional rice field and rice-eel breeding treatments.Samples were collected from the central area and the edge of the ditch in each treatment,namely:conventional fertilization in the central area of rice fields(CCS),conventional fertilization in the ditch edge area of rice fields(CMS),rice-eel breeding model in the central area(ICS),and rice-eel breeding model in the ditch edge area(IMS).The results showed that:compared to conventional rice field treatment,the rice-eel breeding model significantly reduced soil pH and decreased the difference in soil organic matter(SOM)and soil total N(TN)contents between the central and edge areas of the rice field,and increased soil potential nitrification rate(PNR);In comparison to the conventional rice field treatment,the rice-eel breeding model markedly increased the abundance of AOA and Comammox Clade-A and Comammox Clade-B,while significantly decreasing the abundance of AOB.Meanwhile,IMS significantly enhanced the community diversity index of AOA,but reduced the community diversity index of AOB.AOA and Comammox promoted soil nitrification rates,while AOB did the opposite;Soil pH,SOM,and available nitrogen(AN)were identified as key environmental factors influencing ammonia-oxidizing microorganisms(P<0.05);The rice-eel breeding model altered the structure of the ammonia-oxidizing microbial community and enhanced communication among these microorganisms,resulting in a more tightly connected internal network.The rice-eel breeding model,by altering soil environmental factors,especially soil pH,SOM,and AN content,significantly influences the composition,structure,and diversity of soil ammonia-oxidizing microbial communities.It enhances interactions among ammonia-oxidizing microbes and exerts a significant impact on key microbes involved in soil nitrogen cycling.关键词
稻鳝种养/氨氧化微生物/高通量测序/土壤理化性质/微生物群落Key words
rice-eel breeding model/ammonia-oxidizing microorganism/high-throughput sequencing/soil physical and chemical property/microbial community分类
农业科技引用本文复制引用
褚向乾,张翰林,吕卫光,樊海丹,白娜玲,郑宪清,李双喜,张娟琴,张海韵,张月..稻鳝种养模式对土壤氨氧化微生物群落多样性和结构的影响[J].农业环境科学学报,2024,43(6):1350-1359,10.基金项目
上海市现代农业产业技术体系项目[沪农科产字(2022)第3号] （2022）
上海市农业科学院卓越团队建设计划[沪农科卓(2022)008] Agriculture Research System of Shanghai,China(202203) （2022）
Outstanding Team Program of Shanghai Academy of Agricultural Science[Hu-Nong-Ke-Zhuo 2022(008)] （008）
