基于谱效关系筛选广西甜茶调节脂代谢活性质量标志物OA北大核心CSTPCD

Objective:The study was designed to test solvent extracts of Rubus suavissimus(RS)for compounds that regulate lipid metabolism and to measure their relative activity as quantitative markers of lipid metabolism-regulating activities based on the spectrum-effect relationship.Method:RS was extracted with 95％ethanol,the different extraction parts were successively extracted with petroleum ether,methylene chloride,ethyl acetate and n-butanol,respectively.The solution of different extraction parts was applied to the induced differentiated 3T3-L1 preadipocytes,and the oil red O staining and triglyceride release from the cells were determined to screen the best metabolism-regulating site.Analysis of different extraction sited by UPLC-Q-TOF-MS/MS technique to separate and identify the components.Based on specific assays,image intensity analysis,grey correlation and partial least squares regressions,the spectrum-effect relationship of the most abundant active components and their ability to regulate lipid metabolism were determined,and the quality markers were screened.Results:Ethyl acetate and n-butanol extraction site was the best metabolism-regulating site.Four components,including ellagic acid,centaurin-3-O-rutinoside,rubusoside and enantio-kauri-16-ene-19-carboxylic acid-13-O-β-D-glucoside were highly associated with metabolism-regulating effects.It could be used as quality markers for regulating lipid metabolism.Conclusion:Investigation of the quality markers of RS extracts based on the spectrum-effect relationship is of great importance for elucidating the pharmacodynamics,screening the core quality markers for therapeutic activity,and ensuring the safety and rational application of traditional medicines.