食品工业科技2024，Vol.45Issue(13)：17-29,13.DOI:10.13386/j.issn1002-0306.2023060025
基于谱效关系筛选广西甜茶调节脂代谢活性质量标志物
Screening Quality Markers of Regulating Lipid Metabolism Activity of Rubus suavissimus S.Lee Based on Spectral Effect Relationship
摘要
Abstract
Objective:The study was designed to test solvent extracts of Rubus suavissimus(RS)for compounds that regulate lipid metabolism and to measure their relative activity as quantitative markers of lipid metabolism-regulating activities based on the spectrum-effect relationship.Method:RS was extracted with 95％ethanol,the different extraction parts were successively extracted with petroleum ether,methylene chloride,ethyl acetate and n-butanol,respectively.The solution of different extraction parts was applied to the induced differentiated 3T3-L1 preadipocytes,and the oil red O staining and triglyceride release from the cells were determined to screen the best metabolism-regulating site.Analysis of different extraction sited by UPLC-Q-TOF-MS/MS technique to separate and identify the components.Based on specific assays,image intensity analysis,grey correlation and partial least squares regressions,the spectrum-effect relationship of the most abundant active components and their ability to regulate lipid metabolism were determined,and the quality markers were screened.Results:Ethyl acetate and n-butanol extraction site was the best metabolism-regulating site.Four components,including ellagic acid,centaurin-3-O-rutinoside,rubusoside and enantio-kauri-16-ene-19-carboxylic acid-13-O-β-D-glucoside were highly associated with metabolism-regulating effects.It could be used as quality markers for regulating lipid metabolism.Conclusion:Investigation of the quality markers of RS extracts based on the spectrum-effect relationship is of great importance for elucidating the pharmacodynamics,screening the core quality markers for therapeutic activity,and ensuring the safety and rational application of traditional medicines.关键词
广西甜茶/3T3-L1细胞/脂代谢/谱效关系/质量标志物Key words
Rubus suavissimus/3T3-L1 cells/lipid metabolism/spectrum-effect relationship/quality marker分类
轻工纺织引用本文复制引用
樊兰兰,巫秋连,黄万方,姜蔓菁,潘东进,凌洁..基于谱效关系筛选广西甜茶调节脂代谢活性质量标志物[J].食品工业科技,2024,45(13):17-29,13.基金项目
国家自然科学基金(81903918,31660095) （81903918,31660095）
广西自然科学基金(2020GXNSFAA159165) （2020GXNSFAA159165）
广西中医药大学一流学科项目(2019XK117) （2019XK117）
广西中医药大学桂派中医药传承创新团队(2022B005) （2022B005）
广西壮瑶药重点实验开放课题(GXZYZZ2020-12) （GXZYZZ2020-12）
广西高等学校千名中青年骨干教师培育计划. （）
