甜玉米芯多糖纳米银的合成、表征及体外活性研究OA北大核心CSTPCD

Silver nanoparticles(AgNPs)are widely used in the field of biomedicine.However,the synthesis of AgNPs by traditional physical and chemical methods has problems such as pollution,product toxicity,and time-consuming.Therefore,it is extremely important to explore the preparation of AgNPs.Silver nanoparticles(AgNPs)of sweet corncob poly-saccharide(SCP-AgNPs)were successfully synthesized by green method using sweet corncob polysaccharide(SCP)as raw material and the reaction conditions were optimized.The generated AgNPs were characterized by transmission electron microscopy(TEM),X-ray diffraction,X-ray photoelectron spectroscopy and Fourier transform infrared spectroscopy,and their antioxidant and anti-diabetic abilities in vitro were investigated.The results showed that the optimum reaction conditions of SCP-AgNPs were as follows:The concentration of silver nitrate in the solution was 0.75×10-3 mol/L,the pH was 10.0,and the reaction time was 80 min.The prepared SCP-AgNPs were spherical with an average particle size of 13.5 nm.The structure showed a face-centered cubic crystal structure,and Ag was determined to be Ago state.The synthesized SCP-AgNPs had strong free radical scavenging effect in vitro.At the same time,SCP-AgNPs had a good inhibitory effect on the activity of a-glucosidase and a-amylase.SCP-AgNPs has a significant inhibitory effect on key enzymes of diabetes and has potential anti-diabetic activity.It is a nano-drug suitable for nano-biomedical applications and will be widely used in food processing,medicine and other fields in the future.