食品工业科技2024，Vol.45Issue(13)：83-90,8.DOI:10.13386/j.issn1002-0306.2023080044
超声预处理对亚麻籽胶-大豆分离蛋白复合凝胶凝胶特性及结构的影响
Effect of Ultrasonic Pretreatment on the Gel Properties and Structure of Flaxseed Gum-Soybean Protein Isolate Composite Gels
摘要
Abstract
Objective:This study aimed to investigate the impact of different ultrasonic power and time pretreatments on the structures and gel properties of the flaxseed gum and soybean protein isolate composite gel induced by TG enzyme.Methods:The structural characteristics,molecular conformation,and micro-network structures of the composite gel under varying ultrasonic conditions were analyzed using a texture analyzer,laser particle size meter,circular dichroism(CD),fluorescence spectrum,and scanning electron microscope(SEM).Results:The 50％power ultrasound pretreatment for 10 min could significantly improve the hardness,adhesiveness,chewiness,cohesiveness and water-holding property of the composite gel,and reduce its adhesiveness.After 50％power ultrasound pretreatment for 10 min,the α-helix and β-turn content of composite gel proteins decreased,and the content of β-folding and irregular curls increased,and the fluorescence intensity increased,which was conducive to the improvement of the functional properties of the gel.The microstructure of the composite gel became ordered from disorder,the network density increased,the pore diameter became smaller,and the pore wall was thickened.While after increasing the ultrasound power to 80％,the gel properties fluctuated only slightly and the microstructure was destroyed.Conclusion:50％power ultrasound pretreatment for 10 min can significantly improve the gel properties and network structure of TG enzyme cross-linked flaxseed gum-soybean protein isolate composite.关键词
复合凝胶/亚麻籽胶/超声预处理/质构特性/蛋白结构/微观结构Key words
composite gel/flaxseed gum/ultrasonic pretreatment/textural property/protein structure/microstructure分类
轻工业引用本文复制引用
杜童申,刘悦,马骏骅,王馨怡,沈存宽,杨华,颜金鑫..超声预处理对亚麻籽胶-大豆分离蛋白复合凝胶凝胶特性及结构的影响[J].食品工业科技,2024,45(13):83-90,8.基金项目
浙江省重点研发计划项目(2020C02042) （2020C02042）
宁波市公益类科技计划项目(2022S149) （2022S149）
宁波市公益项目(2021S046)) （2021S046）
宁波市公益项目(202002N3114) （202002N3114）
浙江省一流学科"生物工程"学生创新项目(CX2023003) （CX2023003）
浙江省一流学科"生物工程"学生创新项目(CX2022017) （CX2022017）
浙江省教育厅一般科研项目(Y202146900) （Y202146900）
宁波市自然科学基金(2023J299). （2023J299）
