基于网络药理学探讨黄芪甲苷作为保健食品对慢性萎缩性胃炎调节作用OA北大核心CSTPCD

This study used network pharmacology to forecast and evaluate effectiveness in controlling chronic atrophic gastritis,laying the groundwork for its inclusion in functional foods.The target genes of astragaloside Ⅳ that control chronic atrophic gastritis were gathered for this experiment from databases such as Swiss Target Prediction,PharmaMapper,OMIM,etc.The target gene was identified by taking intersections.Based on the STRING database,a protein-protein interaction(PPI)network was created.Based on the centrality of the inter-mediate number,the PPI network did topology analysis using Cytoscape software,and simulated potential molecular docking outcome with Autodock Vina software.GO and KEGG enrichment analysis was conducted on the bioinformatics online platform to screen out relevant sig-nal pathways.In vitro experiments were conducted by inducing GES-1 cells(human gastric mucosal epithelial cells)with MNNG(1-meth-yl-3-nitro-l-nitrosoguanidine)and treating them with astragaloside Ⅳ to detect cell viability and inflammatory factor secretion levels for validation.Finally,53 potential targets were identified for the regulation of chronic atrophic gastritis by astragaloside Ⅳ,including 8 key genes.GO enrichment analysis showed that the vesicular cavity was the main site for biological processes to occur.The KEGG results indi-cated that proteoglycans in tumors were a key signaling pathway for astragaloside Ⅳ to regulate chronic atrophic gastritis.In vitro experi-ments found that astragaloside Ⅳ had a preventive and therapeutic effect on MNNG-induced cell damage,and could downregulate IL-6,IL-8,IL-1β,and TNF-a(P＜0.05).This study showed that astragaloside Ⅳ could operate as a functional factor for the prevention and treatment of chronic atrophic gastritis and could exercise its anti-chronic atrophic gastritis impact through many targets and pathways.