食品与发酵工业2024，Vol.50Issue(12)：284-291,8.DOI:10.13995/j.cnki.11-1802/ts.036270
基于网络药理学探讨黄芪甲苷作为保健食品对慢性萎缩性胃炎调节作用
Intervention of astragaloside Ⅳ on chronic atrophic gastritis based on network pharmacology
包哲隈 1胡舒楠 1杨兰珠 1喻文娟 2杨靖亚3
作者信息
- 1. 上海海洋大学食品学院,上海,201306
- 2. 上海海洋大学水产与生命学院,上海,201306
- 3. 上海海洋大学食品学院,上海,201306||农业部水产品贮藏保鲜质量安全风险评估实验室(上海),上海,201306||国家淡水水产品加工技术研发分中心(上海),上海,201306
- 折叠
摘要
Abstract
This study used network pharmacology to forecast and evaluate effectiveness in controlling chronic atrophic gastritis,laying the groundwork for its inclusion in functional foods.The target genes of astragaloside Ⅳ that control chronic atrophic gastritis were gathered for this experiment from databases such as Swiss Target Prediction,PharmaMapper,OMIM,etc.The target gene was identified by taking intersections.Based on the STRING database,a protein-protein interaction(PPI)network was created.Based on the centrality of the inter-mediate number,the PPI network did topology analysis using Cytoscape software,and simulated potential molecular docking outcome with Autodock Vina software.GO and KEGG enrichment analysis was conducted on the bioinformatics online platform to screen out relevant sig-nal pathways.In vitro experiments were conducted by inducing GES-1 cells(human gastric mucosal epithelial cells)with MNNG(1-meth-yl-3-nitro-l-nitrosoguanidine)and treating them with astragaloside Ⅳ to detect cell viability and inflammatory factor secretion levels for validation.Finally,53 potential targets were identified for the regulation of chronic atrophic gastritis by astragaloside Ⅳ,including 8 key genes.GO enrichment analysis showed that the vesicular cavity was the main site for biological processes to occur.The KEGG results indi-cated that proteoglycans in tumors were a key signaling pathway for astragaloside Ⅳ to regulate chronic atrophic gastritis.In vitro experi-ments found that astragaloside Ⅳ had a preventive and therapeutic effect on MNNG-induced cell damage,and could downregulate IL-6,IL-8,IL-1β,and TNF-a(P＜0.05).This study showed that astragaloside Ⅳ could operate as a functional factor for the prevention and treatment of chronic atrophic gastritis and could exercise its anti-chronic atrophic gastritis impact through many targets and pathways.关键词
黄芪甲苷/慢性萎缩性胃炎/药食同源/网络药理学/分子对接Key words
astragaloside Ⅳ/chronic atrophic gastritis/homologous/network pharmacology/molecular docking引用本文复制引用
包哲隈,胡舒楠,杨兰珠,喻文娟,杨靖亚..基于网络药理学探讨黄芪甲苷作为保健食品对慢性萎缩性胃炎调节作用[J].食品与发酵工业,2024,50(12):284-291,8.
