响应面法优化大鲵肽-硒螯合物制备工艺及其性质研究OA北大核心CSTPCD

To improve the chelating ability of salamander peptide with sodium selenite,the preparation process of salamander pep-tide-selenium chelate was optimized by single factor experiment,Plackett-Burman test,the steepest climb test,and Box-Behnken test.Va-rious spectroscopic methods were used to characterize and analyze the structure characteristics of salamander peptide-selenium chelate,and its in vitro antioxidant activity.Results showed that when the concentration of peptide was 7％,chelation time was 60 min,volume ra-tio was 46∶100,pH was 8.46,and chelation temperature was 65 ℃,the yield of peptide-selenium chelate was 36.18％,which was not significantly different from the predicted value of 34.54％(P＞0.05).The absorption peak intensity of the ultraviolet spectrum increased and redshifted after chelating the peptide with sodium selenite.The intensity and position of the peaks of the—COOH and amide functional groups were changed by Fourier transform infrared spectroscopy.The contents of α-helix and β-folding were significantly increased(P＜0.05),while the contents of β-angle and random curling were significantly decreased(P＜0.05).The peptide-selenium chelates showed the strong scavenging ability of hydroxyl radical and superoxide anion radical and reducing ability.In this study,a novel polypeptide sele-nium chelate was prepared,which could provide basic data for high value utilization of giant salamander peptides.