食品与发酵工业2024，Vol.50Issue(11)：185-194,10.DOI:10.13995/j.cnki.11-1802/ts.035888
响应面法优化大鲵肽-硒螯合物制备工艺及其性质研究
Optimization of preparation process and properties of giant salamander peptide-selenium chelates using response surface methodology
摘要
Abstract
To improve the chelating ability of salamander peptide with sodium selenite,the preparation process of salamander pep-tide-selenium chelate was optimized by single factor experiment,Plackett-Burman test,the steepest climb test,and Box-Behnken test.Va-rious spectroscopic methods were used to characterize and analyze the structure characteristics of salamander peptide-selenium chelate,and its in vitro antioxidant activity.Results showed that when the concentration of peptide was 7％,chelation time was 60 min,volume ra-tio was 46∶100,pH was 8.46,and chelation temperature was 65 ℃,the yield of peptide-selenium chelate was 36.18％,which was not significantly different from the predicted value of 34.54％(P＞0.05).The absorption peak intensity of the ultraviolet spectrum increased and redshifted after chelating the peptide with sodium selenite.The intensity and position of the peaks of the—COOH and amide functional groups were changed by Fourier transform infrared spectroscopy.The contents of α-helix and β-folding were significantly increased(P＜0.05),while the contents of β-angle and random curling were significantly decreased(P＜0.05).The peptide-selenium chelates showed the strong scavenging ability of hydroxyl radical and superoxide anion radical and reducing ability.In this study,a novel polypeptide sele-nium chelate was prepared,which could provide basic data for high value utilization of giant salamander peptides.关键词
大鲵/肽硒螯合物/工艺优化/结构/抗氧化活性Key words
giant salamander/peptide-selenium chelate/process optimization/structure/antioxidant activity引用本文复制引用
刘俊霞,吴艳,赵世博,姜鹏飞,金文刚..响应面法优化大鲵肽-硒螯合物制备工艺及其性质研究[J].食品与发酵工业,2024,50(11):185-194,10.基金项目
陕西理工大学重点科研项目(SLG2106) （SLG2106）
陕西省外国专家服务计划项目(2024WZ-YBXM-40) （2024WZ-YBXM-40）
汉中市青年科技创新团队支持计划(汉科[2019]26号) （汉科[2019]26号）
