食品与发酵工业2024，Vol.50Issue(11)：195-200,6.DOI:10.13995/j.cnki.11-1802/ts.035372
鱼粉压榨液中二十碳五烯酸和二十二碳六烯酸的富集及其甘油酯化
Enrichment and glyceride synthesis of eicosapentaenoic acid and docosahexaenoic from pressed liquor of fish meal processing
摘要
Abstract
Eicosapentaenoic acid(EPA)and docosahexaenoic acid(DHA)were enriched from pressed liquor of fish meal process-ing,and then glyceride was synthesized by immobilized enzyme CALB-A in two steps.Results showed that the total content of EPA and DHA in ethyl ester fish oil enriched by first-order molecular distillation was 43.2％,and the recovery rate was 87.04％.The esterification of ethyl ester fish oil with glycerol was catalyzed by the immobilized enzyme CALB-A to synthesize the glyceride of fish oil.The optimum conditions were that the molar ratio of glycerol to ethyl ester was 1:4,the reaction was carried out in tert-butanol solvent at 50 ℃ for 6 h with a substrate concentration of 20％and enzyme addition of 2％,and then the recovered oil phase was reacted subsequently at 50 ℃ for 24 h under vacuum with enzyme addition of 3％.The conversion rate of ethyl ester and the yield of triglyceride(TAG)were 68.27％and 62.51％,respectively.The analysis of fatty acids showed that the fatty acid composition in TAG was consistent with that of raw materials.关键词
鱼粉压榨液/分子蒸馏/固定化酶/甘油三酯Key words
pressed liquor of fish meal processing/molecular distillation/immobilized enzyme/triglyceride引用本文复制引用
王竞,毕云华,黄佳婷,孟柯,曹少谦,戚向阳..鱼粉压榨液中二十碳五烯酸和二十二碳六烯酸的富集及其甘油酯化[J].食品与发酵工业,2024,50(11):195-200,6.基金项目
宁波市公益性研究计划项目(202002N3073) （202002N3073）
