鱼粉压榨液中二十碳五烯酸和二十二碳六烯酸的富集及其甘油酯化OA北大核心CSTPCD
Enrichment and glyceride synthesis of eicosapentaenoic acid and docosahexaenoic from pressed liquor of fish meal processing
该文从鱼粉压榨液中富集二十碳五烯酸(eicosapentaenoic acid,EPA)和二十二碳六烯酸(docosahexaeno-ic acid,DHA),进而采用固定化酶CALB-A两步法催化合成甘油酯型鱼油.结果表明,经一级分子蒸馏富集的乙酯型鱼油中EPA+DHA含量为43.2％,回收率为87.04％;以固定化酶CALB-A催化该乙酯型鱼油和甘油反应合成甘油酯型鱼油,优化后的条件为,以甘油/乙酯物质的量比1∶4、2％加酶量(以底物总质量计)、底物浓度20％(质量分数)、反应温度50℃的条件在叔丁醇溶剂中反应6 h,回收的油相以3％加酶量(以底物总质量计)、反应温度50 ℃、真空条件下反应24 h,乙酯的转化率可以达到68.27％,甘油三酯(triacylglycerol,TAG)产率为62.51％;脂肪酸分析表明得到TAG中脂肪酸组成与原料一致.
Eicosapentaenoic acid(EPA)and docosahexaenoic acid(DHA)were enriched from pressed liquor of fish meal process-ing,and then glyceride was synthesized by immobilized enzyme CALB-A in two steps.Results showed that the total content of EPA and DHA in ethyl ester fish oil enriched by first-order molecular distillation was 43.2％,and the recovery rate was 87.04％.The esterification of ethyl ester fish oil with glycerol was catalyzed by the immobilized enzyme CALB-A to synthesize the glyceride of fish oil.The optimum conditions were that the molar ratio of glycerol to ethyl ester was 1:4,the reaction was carried out in tert-butanol solvent at 50 ℃ for 6 h with a substrate concentration of 20％and enzyme addition of 2％,and then the recovered oil phase was reacted subsequently at 50 ℃ for 24 h under vacuum with enzyme addition of 3％.The conversion rate of ethyl ester and the yield of triglyceride(TAG)were 68.27％and 62.51％,respectively.The analysis of fatty acids showed that the fatty acid composition in TAG was consistent with that of raw materials.
王竞;毕云华;黄佳婷;孟柯;曹少谦;戚向阳
浙江万里学院生物与环境学院,浙江宁波,315100
鱼粉压榨液分子蒸馏固定化酶甘油三酯
pressed liquor of fish meal processingmolecular distillationimmobilized enzymetriglyceride
《食品与发酵工业》 2024 (011)
195-200 / 6
宁波市公益性研究计划项目(202002N3073)
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