食品与发酵工业2024，Vol.50Issue(12)：299-307,9.DOI:10.13995/j.cnki.11-1802/ts.037257
基于超高效液相色谱-串联质谱技术的黄花菜不同器官萜类代谢物差异分析
Differences analysis of terpenoid metabolites in different organs of Hemerocallis citrina based on ultra performance liquid chromatography-tandem mass spectrometry
摘要
Abstract
Hemerocallis citrina is an edible and medicinal homologous plant,which is rich in many nutrients and bioactive compo-nents.Terpenoids are a kind of important bioactive component in H.citrina.To understand the dominant terpenoid metabolites in H.citrina roots,stems,leaves,and flowers,respectively,this study analyzed the terpenoid metabolites in these 4 organs based on the ultra perform-ance liquid chromatography-tandem mass spectrometry technology of widely targeted metabolomics.A total of 43 terpenoids were identified,including 7 kinds of monoterpenoids(16.2％),18 kinds of ditepenoids(41.9％),16 kinds of triterpene(37.2％),and 2 kinds of trit-erpene saponin(4.7％).Principal component analysis and clustering analysis indicated that the mass spectrometry data were reliable,and the accumulation of terpenoids in different organs was significantly different.The dominant terpenoid metabolites in roots included isopima-radieneone,pimaradienone,abietic acid,scutebarbolide C,and isopimaric acid.The dominant terpenoid metabolites in stems included 3,9-dihydroxy-13(14)-labden-16,15-olide and 9,19-cyclolanost-24-en-3-ol(cycloartenol).The dominant terpenoid metabolites in leaves in-cluded viteagnusin A and ent-3β-acetoxyisopimar-15-8β-ol.The dominant terpenoid metabolites in flowers included vitexilactone and 7-O-coumaroyl-loganic acid.This study listed the relative contents of all terpenoids in roots,stems,leaves,and flowers of H.citrina in detail,which provided the foundation for further investigation of the different usages of various plant organs of H.citrina,and the information is important for the food industry and medical treatment.关键词
黄花菜/器官/萜类/超高效液相色谱-串联质谱/广靶代谢组学Key words
Hemerocallis citrina/organs/terpenoid/ultra performance liquid chromatography-tandem mass spectrometry/widely targeted metabolomics引用本文复制引用
吕虹瑞,武珍珍,洪沙沙,贾慧敏,郭尚..基于超高效液相色谱-串联质谱技术的黄花菜不同器官萜类代谢物差异分析[J].食品与发酵工业,2024,50(12):299-307,9.基金项目
山西农业大学博士科研启动项目(2021BQ121) （2021BQ121）
山西省基础研究计划项目(202203021222168) （202203021222168）
国家重点研发计划项目(2021YFD1600102) （2021YFD1600102）
