基于超高效液相色谱-串联质谱技术的黄花菜不同器官萜类代谢物差异分析OA北大核心CSTPCD

Hemerocallis citrina is an edible and medicinal homologous plant,which is rich in many nutrients and bioactive compo-nents.Terpenoids are a kind of important bioactive component in H.citrina.To understand the dominant terpenoid metabolites in H.citrina roots,stems,leaves,and flowers,respectively,this study analyzed the terpenoid metabolites in these 4 organs based on the ultra perform-ance liquid chromatography-tandem mass spectrometry technology of widely targeted metabolomics.A total of 43 terpenoids were identified,including 7 kinds of monoterpenoids(16.2％),18 kinds of ditepenoids(41.9％),16 kinds of triterpene(37.2％),and 2 kinds of trit-erpene saponin(4.7％).Principal component analysis and clustering analysis indicated that the mass spectrometry data were reliable,and the accumulation of terpenoids in different organs was significantly different.The dominant terpenoid metabolites in roots included isopima-radieneone,pimaradienone,abietic acid,scutebarbolide C,and isopimaric acid.The dominant terpenoid metabolites in stems included 3,9-dihydroxy-13(14)-labden-16,15-olide and 9,19-cyclolanost-24-en-3-ol(cycloartenol).The dominant terpenoid metabolites in leaves in-cluded viteagnusin A and ent-3β-acetoxyisopimar-15-8β-ol.The dominant terpenoid metabolites in flowers included vitexilactone and 7-O-coumaroyl-loganic acid.This study listed the relative contents of all terpenoids in roots,stems,leaves,and flowers of H.citrina in detail,which provided the foundation for further investigation of the different usages of various plant organs of H.citrina,and the information is important for the food industry and medical treatment.