柱前衍生-高效液相色谱法测定巴戟天中氨基酸含量及营养价值评价OA北大核心CSTPCD
Determination of amino acids by pre-column derivatization-high performance liquid chromatography and nutritional evaluation of Morindae officinalis Radix
建立柱前衍生-高效液相色谱法测定巴戟天中氨基酸含量,采用氨基酸比值系数法评价其营养价值.采用盐酸水解法制得巴戟天氨基酸供试品溶液,以异硫氰酸苯酯和三乙胺为柱前衍生化试剂,采用高效液相色谱法,使用Ultimate Amino Acid色谱柱(4.6 mm × 250 mm,5 μm)以0.1 mol/L无水乙酸钠-乙腈为流动相,梯度洗脱,柱温40 ℃,检测波长为254 nm,测定氨基酸含量;通过氨基酸比值、氨基酸比值系数、氨基酸比值系数分等评价其营…查看全部>>
This study aimed to establish a pre-column derivatization-high performance liquid chromatography method for the determi-nation of amino acids in Morindae officinalis Radix and evaluate its nutritional value by amino acid ratio coefficient method.The amino acid test solution of Morindae officinalis Radix was prepared by hydrochloric acid hydrolysis method.Phenyl isothiocyanate and triethylamine were used as pre-column derivatization reagents.The amino acid co…查看全部>>
史娟兰;奉容;高伟城;何丽珊;王小平
漳州卫生职业学院教务处,福建漳州,363000||漳州卫生职业学院药学院,福建漳州,363000厦门医学院药学系,福建厦门,361023漳州卫生职业学院药学院,福建漳州,363000漳州卫生职业学院药学院,福建漳州,363000漳州卫生职业学院药学院,福建漳州,363000
巴戟天柱前衍生-高效液相色谱法氨基酸营养价值评价系统聚类分析主成分分析
Morindae officinalis Radixpre-column derivatization-high performance liquid chromatographyamino acidnutritional evalu-ationsystematic cluster analysisprincipal component analysis
《食品与发酵工业》 2024 (12)
308-318,11
福建省中青年教师教育科研项目(科技类)(JAT201402)漳州市市级个人科技特派员资助项目(20210285)漳州卫生职业学院科技创新团队培育计划项目(Kjcx-5)漳州卫生职业学院科研项目(ZWYZ202006)
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