食品与发酵工业2024，Vol.50Issue(12)：308-318,11.DOI:10.13995/j.cnki.11-1802/ts.037008
柱前衍生-高效液相色谱法测定巴戟天中氨基酸含量及营养价值评价
Determination of amino acids by pre-column derivatization-high performance liquid chromatography and nutritional evaluation of Morindae officinalis Radix
摘要
Abstract
This study aimed to establish a pre-column derivatization-high performance liquid chromatography method for the determi-nation of amino acids in Morindae officinalis Radix and evaluate its nutritional value by amino acid ratio coefficient method.The amino acid test solution of Morindae officinalis Radix was prepared by hydrochloric acid hydrolysis method.Phenyl isothiocyanate and triethylamine were used as pre-column derivatization reagents.The amino acid content was determined by high-performance liquid chromatography with Ultimate Amino Acid chromatographic column(4.6 mm × 250 mm,5 μm),0.1 mol/L anhydrous sodium acetate-acetonitrile as mobile phase,gradient elution,column temperature of 40 ℃,and detection wavelength of 254 nm.The nutritional value of amino acids was eval-uated by amino acid ratio(RAA),amino acid ratio coefficient(RC),and ratio coefficient score(SRC).Systematic cluster analysis and principal component analysis were used in classification analysis.The linear relationship of 15 amino acids was good,and the correlation coefficient r≥ 0.999 6.The total amino acid contents of 16 batches of Morindae officinalis Radix were between 17.87 mg/g and 102.15 mg/g.Fifteen kinds of amino acids were detected in all 16 samples,including 6 kinds of essential amino acids,accounting for 14.15％to 32.06％,and 8 kinds of medicinal amino acids,accounting for 45.20％to 56.31％.The limiting amino acids were threonine and leucine.The amino acid ratio coefficient was between 33.87 and 61.31.The correlation of amino acid composition of Morindae offici-nalis Radix was significant.Two principal components were proposed in principal component analysis,and the contribution rate of cumula-tive variance was 97.085％.16 batches of Morindae officinalis Radix are divided into 3 categories.This method was simple and effective.It could effectively separate 15 kinds of amino acids,and had good precision and repeatability.It has high medicinal value and can provid-ed a reference for quality evaluation and resource development of Morindae officinalis Radix.关键词
巴戟天/柱前衍生-高效液相色谱法/氨基酸/营养价值评价/系统聚类分析/主成分分析Key words
Morindae officinalis Radix/pre-column derivatization-high performance liquid chromatography/amino acid/nutritional evalu-ation/systematic cluster analysis/principal component analysis引用本文复制引用
史娟兰,奉容,高伟城,何丽珊,王小平..柱前衍生-高效液相色谱法测定巴戟天中氨基酸含量及营养价值评价[J].食品与发酵工业,2024,50(12):308-318,11.基金项目
福建省中青年教师教育科研项目(科技类)(JAT201402) （科技类）
漳州市市级个人科技特派员资助项目(20210285) （20210285）
漳州卫生职业学院科技创新团队培育计划项目(Kjcx-5) （Kjcx-5）
漳州卫生职业学院科研项目(ZWYZ202006) （ZWYZ202006）
