食品工业科技2024，Vol.45Issue(13)：212-220,9.DOI:10.13386/j.issn1002-0306.2023120305
苦参多糖的提取工艺优化及体外抗氧化、降血脂活性分析
Optimization of the Extraction Process and in Vitro Antioxidant and Hypolipidemic Activities of Polysaccharides from Sophora flavescens
摘要
Abstract
Objective:To increase the yield of Sophora flavescens polysaccharides and elucidate their antioxidant and hypolipidemic properties,this study optimised the polysaccharide extraction process through orthogonal experiments,followed by evaluation of their antioxidant and hypolipidemic activities in vitro.Methods:Using polysaccharide yield as the primary metric,this study performed orthogonal experiments to optimise the extraction process,building on preliminary one-way experiments.This study investigated the effect of extraction temperature,number of extractions,material-liquid ratio and extraction time on the polysaccharide yield of Sophora flavescens.Furthermore,this study evaluated the in vitro antioxidant activity of the polysaccharides by investigating their scavenging potential against DPPH radicals,hydroxyl radicals and superoxide anion radicals.In addition,the in vitro hypolipidemic activity of the polysaccharides was evaluated by their binding affinity to sodium cholate(SC),sodium taurocholate(STC),sodium glycylcholate(SGC)and mixed bile salts(MBS).Results:The optimal conditions for the extraction of Sophora flavescens polysaccharide were determined as follows:a temperature of 85 ℃,a material-liquid ratio of 1∶35(g∶mL),and an extraction duration of 3.5 hours,yielding an average polysaccharide content of 3.07％±0.07％.The scavenging efficiencies of Sophora flavescens polysaccharides against DPPH radicals,hydroxyl radicals,and superoxide anion radicals at a concentration of 5 mg/mL were measured at 60.1％±3.6％,39.6％±3.1％and 45.1％±4.1％,respectively.Furthermore,the binding affinities of Sophora flavescens polysaccharides to SC,STC,SGC and MBS at a concentration of 10 mg/mL were determined to be 34.4％±0.75％,45.4％±0.15％,19.7％±0.94％and 37.7％±0.64％,respectively.Conclusion:Sophora flavescens polysaccharide exhibits notable in vitro antioxidant and hypolipidemic activities.This investigation furnishes a foundational understanding for the potential development and exploitation of Sophora flavescens polysaccharide.关键词
苦参/多糖/工艺优化/抗氧化活性/降血脂活性Key words
Sophora flavescens Ait./polysaccharide/process optimized/antioxidant activity/hypolipidemic activity分类
轻工纺织引用本文复制引用
赵小亮,牟翮,张晶,张伟杰..苦参多糖的提取工艺优化及体外抗氧化、降血脂活性分析[J].食品工业科技,2024,45(13):212-220,9.基金项目
国家自然科学基金项目(32260231,31860250) （32260231,31860250）
甘肃省高校教师创新基金项目(2023A-020) （2023A-020）
兰州市青年科技人才创新项目(2023-QN-65). （2023-QN-65）
