食品与发酵工业2024，Vol.50Issue(12)：362-369,8.DOI:10.13995/j.cnki.11-1802/ts.036728
基于转录因子的生物传感器研究进展
Research progress on transcription-factor-based biosensors
摘要
Abstract
Transcription-factor-based biosensors(TFBBs),as an important tool in synthetic biology,play an important role in high throughput screening,adaptive evolution,dynamic regulation,and so on.However,natural TFBBs cannot meet the current application re-quirements due to its low sensitivity,poor specificity,and slow response.To cope with the above challenges,this paper briefly introduces the classification and principle of TFBBs,and focuses on the performance regulation strategies of TFBBs,including the optimization of sensitivity,specificity,detection range and dynamic range of biosensors by using strategies such as directed evolution and protein engi-neering.The applications of TFBBs in high-throughput screening,adaptive evolution and dynamic regulation are summarized.The potential opportunities and challenges of TFBBs in practical applications are discussed,and the development trend of TFBBS is prospected.It is pointed out that the rapid development of synthetic biology will promote the application of biosensors in a wider range of fields.关键词
转录因子的生物传感器/性能调节/高通量筛选/适应性进化/动态调控Key words
transcription-factor-based biosensors/performance regulation/high-throughput screening/adaptive evolution/dynamic regu-lation引用本文复制引用
赵梅,罗佳璐,王震,周海星,杨爽黎,商洁,齐向辉..基于转录因子的生物传感器研究进展[J].食品与发酵工业,2024,50(12):362-369,8.基金项目
国家自然科学基金项目(32200061,32272284) （32200061,32272284）
江苏省自然科学基金项目(BK20210752) （BK20210752）
中国博士后项目(2023T160278,2022M721390) （2023T160278,2022M721390）
