食品工业科技2024，Vol.45Issue(13)：221-229,9.DOI:10.13386/j.issn1002-0306.2023050242
固相萃取-超高效液相色谱-串联质谱同时测定乳制品中的苯并咪唑类药物及其代谢物残留量
Simultaneous Determination of Benzimidazoles and Their Metabolites Residues in Dairy Products by Solid Phase Extraction-Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry
摘要
Abstract
A method for the simultaneous determination of benzimidazoles and their metabolites in dairy products was established using ultra high performance liquid chromatography-tandem mass spectrometry(MS).After extracting the samples with acetonitrile containing 0.2％formic acid,the extracts were centrifuged and purified by solid phase extraction column,and then separated by UPLC HSS T3 chromatographic column,determined in multi reaction monitoring(MRM)method with a positive ESI mode.The results showed that the linear correlation coefficient(r)within the range of 1.0～50 pg/L was greater than 0.995(except for triclabendazole,which was 0.9578).The method had a limit of detection(LOD)(S/N=3)of 0.002 mg/kg(except for albendazole-2-aminosulfone,thiabendazole,and thiabendazole-5-hydroxy,which were 0.003 mg/kg).The limit of quantitation(LOQ)(S/N=10)was 0.005 mg/kg(except for albendazole-2-aminosulfone,thiabendazole,and thiabendazole-5-hydroxy,which were 0.010 mg/kg).The recoveries at three spiked levels of 0.005,0.01 and 0.05 mg/kg ranged from 62.5％to 117.6％,with relative standard deviations(RSD)between 1.92％and 12.17％.The method is simple to operate,highly sensitive,and suitable for the quantitative analysis of benzimidazoles and their metabolites in dairy products.关键词
固相萃取/超高效液相色谱-串联质谱/苯并咪唑类药物及其代谢物/乳制品/残留量测定Key words
solid phase extraction/ultra-performance liquid chromatography-tandem mass spectrometry/benzimidazoles and their metabolites/dairy products/determination of residue分类
轻工纺织引用本文复制引用
乔勇升,王萍,黄银波,冯寅洁,蔡霞,张占林,陈伟..固相萃取-超高效液相色谱-串联质谱同时测定乳制品中的苯并咪唑类药物及其代谢物残留量[J].食品工业科技,2024,45(13):221-229,9.基金项目
江苏省市场监督管理局科技计划项目(KJ21125052). （KJ21125052）
