食品工业科技2024，Vol.45Issue(13)：265-271,7.DOI:10.13386/j.issn1002-0306.2023080334
利用时间分辨荧光免疫层析法同时定量检测玉米中的伏马毒素B1、B2和B3
Application of Time-resolved Fluorescence Immunoassay to Concurrently Quantify Fumonisin B1,B2 and B3 in Corn
张佳林 1马立才 2侯璐 3孟和那仁 4温凯 5侯晓林3
作者信息
- 1. 北京农学院动物科学技术学院,北京 102206||北京维德维康生物技术有限公司,北京 100095
- 2. 北京维德维康生物技术有限公司,北京 100095
- 3. 北京农学院动物科学技术学院,北京 102206
- 4. 赤峰市农牧业综合行政执法支队,内蒙古赤峰 024000
- 5. 中国农业大学动物医学院,北京 100193
- 折叠
摘要
Abstract
This study aimed to establish a time-resolved fluorescence immunoassay method for the simultaneous quantitative detection of fumonisin B1,B2 and B3 in corn.The EDC/NHS active ester method was used to prepare time-resolved fluorescent microspheres and fumonisin antibody conjugates.Fumonisin antigen was immobilized on a nitrocellulose membrane as the test line.By optimizing the number of test line,a competitive immunoassay method for detecting fumonisin was eventually established.Subsequently,the sensitivity,specificity,accuracy,precision,and correlation with the national standard method were evaluated for the established method.The detection limits of the established method by using two test line pattern were 107.68～168.28 pg/kg,and the quantification limits were 283.46～444.63 pg/kg.The cross-reactivity with fumonisin B1,fumonisin B2 and fumonisin B3 were 100％,85.59％and 72.72％,respectively,and no significant cross-reactivity was observed with five other common mycotoxins.The recovery rates ranged from 88.37％to 117.42％,and the coefficients of variation were lower than 10％.The conformity of the results obtained by the established method,as compared to the immunoaffinity column purification-high performance liquid chromatography(IAC-HPLC)method specified in the national standard GB 5009.240-2016,fell within the range of 92.17％to 107.21％.The established time-resolved fluorescence immunoassay method meets the requirements for rapid on-site quantitative detection of fumonisin B1,B2 and B3 in corn.关键词
时间分辨荧光微球/伏马毒素B1/伏马毒素B2/伏马毒素B3/玉米/定量检测Key words
time-resolved fluorescent microsphere/fumonisin B1/fumonisin B2/fumonisin B3/corn/quantitative detection分类
轻工纺织引用本文复制引用
张佳林,马立才,侯璐,孟和那仁,温凯,侯晓林..利用时间分辨荧光免疫层析法同时定量检测玉米中的伏马毒素B1、B2和B3[J].食品工业科技,2024,45(13):265-271,7.
