食品工业科技2024，Vol.45Issue(13)：272-280,9.DOI:10.13386/j.issn1002-0306.2023090167
高效液相色谱法测定大果山楂果实中6种三萜酸类成分的含量
Determination of Content of 6 Triterpenic Acids in Malus doumeri Fruit by High-performance Liquid Chromatography
摘要
Abstract
A HPLC method was developed for the simultaneous determination of euscaphic acid,2α,19α-dihydroxy-3-oxo-urs-12-en-28-oic acid,maslinic acid,corosolic acid,oleanic acid,and ursolic acid in Malus doumeri fruits.Analyses were conducted on the distribution and content of these six triterpenic acids in Malus doumeri fruits from six producing areas in Guangxi.Using gradient elution at a flow rate of 0.8 mL/min,a Diamonsil C18(2)column was used with a mobile phase of 0.1％formic acid aqueous solution/methanol(v/v).The detection wavelength was 210 nm and the column temperature was 30 ℃.The results showed that the mass concentrations of the six triterpenic acids had a good linear relationship with the chromatographic peak areas in the linear range.The correlation coefficients were greater than 0.9990,the detection limits were within 0.46～2.00 μg/mL,the lower quantitation limits were within 1.53～6.67 μg/mL,the coefficients of variation were less than 5.0％,and the recovery percentages were within 99.2％～101.3％.Six samples from different production areas contained six triterpenic acids(euscaphic acid,2a,19a-dihydroxy-3-oxo-urs-12-en-28-oic acid,maslinic acid,corosolic acid,oleanic acid,and ursolic acid).Each of the six triterpenic acids was a pentacyclic triterpenic acid.Among the six triterpenic acids,euscaphic acid and 2α,19α-dihydroxy-3-oxo-urs-12-en-28-oic acid were first detected in Malus doumeri fruits.Ursolic acid,euscaphic acid,and 2α,19α-dihydroxy-3-oxo-urs-12-en-28-oic acid were predominant,accounting for 71.82％～81.38％of the total triterpenic acids.The total triterpenic acids content ranged from 808.74 to 1090.75 pg/g FM.The total triterpenic acids content of fruits from Liujiang district of Liuzhou and Pingle of Guilin were relatively high,reaching 1090.75 pg/g FM and 1045.20 pg/g FM,respectively.Overall,this study's HPLC method can detect the triterpenic acids content of Malus doumeri fruits and provide scientific references for the quality evaluation of Malus doumeri fruit's raw materials and products.关键词
HPLC/大果山楂/果实/三萜酸/含量Key words
HPLC/Malus doumeri/fruits/triterpenic acids/content分类
轻工纺织引用本文复制引用
霍华珍,蔡爱华,谢运昌..高效液相色谱法测定大果山楂果实中6种三萜酸类成分的含量[J].食品工业科技,2024,45(13):272-280,9.基金项目
广西科技重大专项(桂科AA17204038) （桂科AA17204038）
广西自然科学基金(2017GXNSFAA198009) （2017GXNSFAA198009）
广西植物功能物质与资源持续利用重点实验室主任基金(ZRJJ2022-7,ZRJJ2020-3,ZRJJ2018-2) （ZRJJ2022-7,ZRJJ2020-3,ZRJJ2018-2）
广西植物研究所基本业务费(桂植业21004,桂植业22001) （桂植业21004,桂植业22001）
桂林市创新平台和人才计划(20210102-3,20220124-13). （20210102-3,20220124-13）
