食品工业科技2024，Vol.45Issue(13)：281-291,11.DOI:10.13386/j.issn1002-0306.2023090309
甘肃省陇中地区小米游离氨基酸组成及综合评价
Composition and Comprehensive Evaluation of Free Amino Acids in Millet from Longzhong Area in Gansu Province
摘要
Abstract
To explore the differences in the composition,content and comprehensive quality of free amino acids(FAA)in millet from Longzhong area in Gansu Province,12 different cultivars of millet were used as research objects,the types and contents of FAA were determined by liquid chromatography-tandem mass spectrometry.Comprehensive evaluation of FAA quality was performed by principal component analysis(PCA)and hierarchical cluster analysis(HCA).The results showed that 19～20 kinds of FAA were detected in all kinds of millet,and the total content of FAA ranged from 1046.80～1773.85 mg/kg,asparagine(Asn),aspartic acid(Asp),glutamic acid(Glu)and glycine(Gly)were the four main FAA in millet.There were significant differences in the contents of FAA(P＜0.05),but no obvious difference in types in the 12 cultivars of millet.The characteristics of flavor amino acids in millet were delicious amino acids＞bitter amino acids＞sweet amino acids＞aromatic amino acids.9 Medicinal amino acids and 3 branched chain amino acids were found in millet,the average content of medicinal amino acids was 706.34 mg/kg,the percentages of medicinal amino acids to total FAA was 53.63％,so millet had many potential medicinal values.There was a significant relation with FAA in different cultivars of millet,five principal components were extracted after PCA,and the cumulative contribution ratio of the five components was 86.20％,the FAA comprehensive scores of LG-23、LG-11 and YG-18 were ranked as the top three.The 12 different cultivars millet were divided into four groups by HCA,FAA comprehensive quality performances of groups Ⅰ(LG-23)and Ⅲ(LG-11 and YG-18)were optimal,the content of total FAA was significantly higher than the other two groups(P＜0.05),but group Ⅱ(HNLG、LG-18、LG-029)had relatively poor FAA quality,and the results were consistent with the PCA,which reflected the distinction among different cultivars of millet.关键词
小米/游离氨基酸/主成分分析/聚类分析/综合评价Key words
millet/free amino acids/principal component analysis/cluster analysis/comprehensive evaluation分类
轻工纺织引用本文复制引用
柳利龙,张爱琴,徐瑞,郭斌,王青..甘肃省陇中地区小米游离氨基酸组成及综合评价[J].食品工业科技,2024,45(13):281-291,11.基金项目
甘肃省农业科学院中青年基金(2023GAAS28,2022GAAS49) （2023GAAS28,2022GAAS49）
甘肃省青年科技基金计划项目(20JR10RA462) （20JR10RA462）
甘肃省农业农村厅科技项目(GNKJ-2020-30). （GNKJ-2020-30）
