食品与机械2024，Vol.40Issue(5)：67-72,160,7.DOI:10.13652/j.spjx.1003.5788.2023.80802
保健食品中7种烟酰胺类化合物含量测定
Determination of seven nicotinamide compounds in health products
摘要
Abstract
Objective:This study aimed to establish an ultra-performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS)method for simultaneous determination of 7 nicotinamide compounds in health food.Methods:The samples were dissolved in 10％methanol water,extracted by ultrasonic,and 10 mmol/L ammonium acetate-acetonitrile was used as the mobile phase of gradient elution.The seven nicotinamide compounds were monitored by ESI,positive and negative ion scanning mode,and multiple reaction monitoring(MRM)mode.Results:The 7 nicotinamide compounds showed good linearity in the mass concentration range,and the correlation coefficients were greater than 0.996.The limit of detection(LOD)was 0.075～0.600 mg/kg,the recovery was 84.6％～108.6％with the relative standard deviation of 2.1％～8.7％(n=6).Conclusion:The method has the advantages of simple operation,high speed,high efficiency,high recovery rateand good precision,and can be used for qualitative and quantitative analysis of nicotinamide and other analogues in health care foods.关键词
保健食品/烟酰胺类化合物/超高效液相色谱—串联质谱法/多反应监测/同时测定Key words
health care foods/nicotinamide compounds/ultra-high performance liquid chromatography-tandem mass spectrometry/multiple reaction monitoring mode/simultaneous determination引用本文复制引用
吴滋灵,何敏恒,黄嘉乐,蔡夏怡,孙雯雯..保健食品中7种烟酰胺类化合物含量测定[J].食品与机械,2024,40(5):67-72,160,7.基金项目
广东省市场监督管理局科技项目(编号:2022CS03) （编号:2022CS03）
