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分子生物学技术在食源性致病菌快速检测中的研究进展

张德福 高乐 张明 杜知心 吕欣然 柏雪 张国清 李学鹏 励建荣

食品工业科技2024,Vol.45Issue(13):368-377,10.
食品工业科技2024,Vol.45Issue(13):368-377,10.DOI:10.13386/j.issn1002-0306.2023080029

分子生物学技术在食源性致病菌快速检测中的研究进展

Research Progress of Molecular Biological Technology in Rapid Detection of Foodborne Pathogens

张德福 1高乐 1张明 1杜知心 1吕欣然 1柏雪 1张国清 2李学鹏 1励建荣1

作者信息

  • 1. 渤海大学食品科学与工程学院,渤海大学海洋研究院,生鲜农产品贮藏加工及安全控制技术国家地方联合工程研究中心,辽宁锦州 121013
  • 2. 锦州市农业农村综合服务中心水产技术推广站,辽宁锦州 121013
  • 折叠

摘要

Abstract

Foodborne pathogens are one of the important factors affecting food safety.Efficient and rapid detection technology of foodborne pathogens is particularly important to ensure food safety.Among the rapid detection methods of foodborne pathogens,molecular biology technology has been widely used because of its advantages of strong specificity,high accuracy,and fast detection speed.This paper introduces the application of various molecular biological techniques in the rapid detection of foodborne pathogens,including polymerase chain reaction(PCR),loop-mediated isothermal amplification(LAMP),nuclear acid sequence-based amplification(NAS-BA),recombinase polymerase amplification(PRA),recombinase-aided isothermal amplification(RAA),and gene chip(GC).The application status,application scope and existing problems of these technologies are also analyzed and summarized to provide theoretical reference for the rapid detection of foodborne pathogens in the future.

关键词

分子生物学技术/食源性致病菌/快速检测/食品安全

Key words

molecular biology technology/foodborne pathogens/rapid detection/food safety

分类

轻工纺织

引用本文复制引用

张德福,高乐,张明,杜知心,吕欣然,柏雪,张国清,李学鹏,励建荣..分子生物学技术在食源性致病菌快速检测中的研究进展[J].食品工业科技,2024,45(13):368-377,10.

基金项目

渤海大学海洋研究院开放课题(BDHYYJY2024003). (BDHYYJY2024003)

食品工业科技

OA北大核心CSTPCD

1002-0306

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