|国家科技期刊平台
| 注册
首页|期刊导航|食品与发酵工业|基于转录组学分析揭示蜡样芽胞杆菌(Bacillus cereus)AR1002阻控黄曲霉生长的生理机制研究

基于转录组学分析揭示蜡样芽胞杆菌(Bacillus cereus)AR1002阻控黄曲霉生长的生理机制研究

唐晓倩 张靖博 高尚 姜俊 张奇 李培武

食品与发酵工业2024，Vol.50Issue(11)：1-8,8.
✕
食品与发酵工业2024，Vol.50Issue(11)：1-8,8.DOI:10.13995/j.cnki.11-1802/ts.038530

基于转录组学分析揭示蜡样芽胞杆菌(Bacillus cereus)AR1002阻控黄曲霉生长的生理机制研究

Physiological mechanism of Bacillus cereus AR1002 inhibiting growth of Aspergillus flavus based on transcriptomic analysis

唐晓倩 1张靖博 2高尚 3姜俊 4张奇 5李培武6

作者信息

  • 1. 中国农业科学院油料作物研究所,湖北武汉,430062||农业农村部油料及制品质量监督检验测试中心,湖北武汉,430062||农业农村部油料产品质量安全风险评估实验室(武汉),湖北武汉,430062||农业农村部油料作物生物学与遗传育种重点实验室,湖北武汉,430062||湖北大学食品安全研究院,湖北武汉,430062||郑州大学农学院,河南郑州,450000||长江大学,湖北荆州,434025||湘湖实验室,浙江杭州,311231
  • 2. 中国农业科学院油料作物研究所,湖北武汉,430062||郑州大学农学院,河南郑州,450000
  • 3. 中国农业科学院油料作物研究所,湖北武汉,430062||长江大学,湖北荆州,434025
  • 4. 中国农业科学院油料作物研究所,湖北武汉,430062||湖北大学食品安全研究院,湖北武汉,430062
  • 5. 中国农业科学院油料作物研究所,湖北武汉,430062||农业农村部油料及制品质量监督检验测试中心,湖北武汉,430062||农业农村部油料产品质量安全风险评估实验室(武汉),湖北武汉,430062||农业农村部油料作物生物学与遗传育种重点实验室,湖北武汉,430062||湖北大学食品安全研究院,湖北武汉,430062||郑州大学农学院,河南郑州,450000||湘湖实验室,浙江杭州,311231||湖北洪山实验室,湖北武汉,430070
  • 6. 中国农业科学院油料作物研究所,湖北武汉,430062||农业农村部油料及制品质量监督检验测试中心,湖北武汉,430062||农业农村部油料产品质量安全风险评估实验室(武汉),湖北武汉,430062||农业农村部油料作物生物学与遗传育种重点实验室,湖北武汉,430062||湖北大学食品安全研究院,湖北武汉,430062||郑州大学农学院,河南郑州,450000||长江大学,湖北荆州,434025||湘湖实验室,浙江杭州,311231||湖北洪山实验室,湖北武汉,430070
  • 折叠

摘要

Abstract

Aspergillus flavus is susceptible to contaminating agricultural food,especially the oilcrops,aflatoxin is the secondary me-tabolite of A.flavus,which is seriously harmful to human health.Preventing and controlling aflatoxin contamination has become an urgent international problem.This study aimed to investigate the physiological and molecular mechanism of Bacillus cereus AR 1002 in inhibiting the growth and pollution of A.flavus.In this study,the metabolites prepared by AR1002 were used to treat A.flavus and then identify its growth and sporulation phenotypes.At the same time,the antibacterial mechanism of AR 1002 was studied by methods of microstructure and transcriptomic analysis.The metabolites of B.cereus AR 1002 could significantly inhibit the mycelium growth,dry matter accumulation and spore number of A.flavus,with inhibition rates of 24.73％,65.00％,and 98.80％,respectively.In addition,AR1002 metabolites can disturb the glycerophosphate metabolism of A.flavus by inhibiting the expression of key genes such as glpA and AYR1.At the same time,the expression of SEC61A,RAD23,ATP13A1,UBE2G2 and other key genes is inhibited to regulate the processing of proteins in the endoplasmic reticulum.AR1002 metabolites also inhibited homologous recombination and non-homologous end-linking processes of A.fla-vus by down-regulating genes related to DNA repair mechanisms such as RAD51 and RAD54B.Finally,the aim of inhibiting the growth and sporulation of A.flavus was achieved.Results indicated that the metabolites of B.cereus AR 1002 were expected to be applied in practical production to solve the problem of A.flavus contamination.

关键词

蜡样芽孢杆菌/代谢物/黄曲霉毒素,转录组学/抑菌机制

Key words

Bacillus cereus/metabolite/aflatoxins/transcriptomics/inhibitory mechanism

引用本文复制引用

唐晓倩,张靖博,高尚,姜俊,张奇,李培武..基于转录组学分析揭示蜡样芽胞杆菌(Bacillus cereus)AR1002阻控黄曲霉生长的生理机制研究[J].食品与发酵工业,2024,50(11):1-8,8.

基金项目

国家重点研发计划项目(2023YFF1104600) （2023YFF1104600）

国家自然科学基金项目(32102089) （32102089）

食品与发酵工业

OA北大核心CSTPCD

0253-990X

访问量0
|
下载量0
段落导航相关论文