食品与发酵工业2024，Vol.50Issue(12)：46-52,62,8.DOI:10.13995/j.cnki.11-1802/ts.037166
基于纳米抗体建立icELISA检测乳制品中黄曲霉毒素M1
Nanobody-based icELISA for aflatoxin M1 in dairy products
摘要
Abstract
Nanobody has become an important tool for immunoassays because of its stability,specificity,and ease of expression.To monitor the aflatoxin M1 in dairy products,the anti-AFM1 nanobody,named M6,selected from the Bactrian camel AFM1 immune librar-y,evaluated its properties,and developed an indirect competitive enzyme-linked immunosorbent assay(icELISA).After optimizing the parameters,including the blocking method,methanol concentrations,pH values,and ionic strength in the reaction system,a standard competitive inhibition curve was established,and methodological specificity and accuracy was verified.Results showed that Nb-M6 had a good affinity and thermal stability,the limit of detection(LOD)of Nb-icELISA was 0.111 ng/mL,and the half maximal inhibitory con-centration(IC50)was 1.498 ng/mL.The recoveries of dairy products spiked were 89.8％-104.1％,which were in agreement with the high-performance liquid chromatography(HPLC).The Nb-icELISA is simple,convenient and can be applied to batch screening of AFM1 in dairy products.关键词
纳米抗体/间接竞争酶联免疫吸附测定法/黄曲霉毒素M1/乳制品Key words
nanobody/indirect competitive enzyme-linked immunosorbent/aflatoxin M1/dairy products引用本文复制引用
刘海媛,刘颖达,Tuyatsetseg Jambal,吉日木图,伊丽..基于纳米抗体建立icELISA检测乳制品中黄曲霉毒素M1[J].食品与发酵工业,2024,50(12):46-52,62,8.基金项目
内蒙古农业大学食品科学与工程学院科技计划项目(SPKJ201913) （SPKJ201913）
内蒙古自治区自然科学基金(2020BS03011) （2020BS03011）
国家重点研发计划项目(2020YFE0203300) （2020YFE0203300）
内蒙古自治区科技成果转化专项资金项目(2021CG0021) （2021CG0021）
