食品与发酵工业2024，Vol.50Issue(12)：135-141,7.DOI:10.13995/j.cnki.11-1802/ts.036805
基于纯培养法结合高通量测序技术解析胀袋辐照凤爪中的微生物多样性
Analysis of microbial diversity in swollen irradiated chicken feet based on pure culture method and high-throughput sequencing technology
摘要
Abstract
To investigate the microbial diversity of vacuum-packaged swollen irradiated chicken feet,the microbial diversity was ana-lyzed in this study using the pure culture method and high-throughput sequencing technology.The pure culture approach revealed the iden-tification of 13 strains,comprising 4 strains of Lactiplantibacillus plantarum,3 strains of Lactobacillus brevis,1 strain of Stenotrophomonas maltophilia,3 strains of Leuconostoc fallax,and 2 strains of Candida tropicalis.The results of high-throughput sequencing showed that Lac-tobacillus exhibited the highest relative abundance(averaging at 97.30％)and was the dominant bacterium in the swollen chicken feet.Additionally,gas production verification experiments revealed that L.brevis and L.fallax could cause bag swelling.The results of this study can provide a certain reference for mastering the microbial contamination and community composition of swollen irradiated chicken feet products,and provide a solid theoretical foundation for the subsequent control of microbial contamination and enhancement of product quality.关键词
胀袋/辐照凤爪/纯培养法/高通量测序/微生物多样性Key words
swollen/irradiated chicken feet/pure culture method/high throughput sequencing/microbial diversity引用本文复制引用
贾鑫,高鹏,周瑞,徐攀,王丹,黄敏,孟爱莲,张雁君,吴舸洋..基于纯培养法结合高通量测序技术解析胀袋辐照凤爪中的微生物多样性[J].食品与发酵工业,2024,50(12):135-141,7.基金项目
四川省重大科技专项(2019ZDZX0003) （2019ZDZX0003）
四川省自然科学基金项目(面上项目)(2023NSFSC0133) （面上项目）
四川省科技创新基地(平台)基本科研业务(2023JDKY0009) （平台）
