中国动物检疫2024，Vol.41Issue(6)：50-55,6.DOI:10.3969/j.issn.1005-944X.2024.06.009
国内部分动物园与花鸟市场鹦鹉热衣原体病原学检测
Pathogenic Detection of Chlamydia psittaci in Zoos and Flower-bird Markets in China
摘要
Abstract
In order to investigate the prevalence of Chlamydia psittaci(Cps)in bird flocks in some zoos and flower-bird markets in China,168 avian fecal samples collected from three sampling sites in Jiangsu Province and Beijing were detected using Taq Man fluorescence quantitative PCR,and gene sequence analysis was conducted for positive samples based on OmpA gene.The results showed that 31 Cps-positive samples were detected in a flower-bird market in Jiangsu Province,with an individual positive rate of 41.3%(31/75),but none was in the other two sampling sites(zoos);the positive samples were from budgerigars(23),peafowl parrots(5),gentoo parrots(2)and perching birds(1).For the positive samples,18 OmpA gene fragments were amplified,in which 16 samples had the same gene sequence,that were not in the same branch as the gene sequence in other two positive samples,but all of which belonged to genotype A.In conclusion,the risk of Cps prevalence in the flower-bird markets was relatively high,with concentrated sources of infection.Cps should be further prevented and controlled in local flower-bird markets,relevant regulatory measures should be implemented to improve quarantine supervision,aiming to reduce its transmission rate and safeguard public health safety.关键词
鹦鹉热衣原体/核酸检测/qPCR/基因进化树/遗传分析Key words
Cps/nucleic acid detection/qPCR/gene evolution tree/genetic analysis分类
农业科技引用本文复制引用
刘心仪,刘燕,代蕾,刘广锦,包银莉,张释丹,陆浩天,文洪然,陈柳蓉,张钲浩,李金飞,陈蓉..国内部分动物园与花鸟市场鹦鹉热衣原体病原学检测[J].中国动物检疫,2024,41(6):50-55,6.基金项目
南京农业大学三亚研究院引导资金项目(NAUSY-MS21) （NAUSY-MS21）
海南省重点研发项目(ZDYF2022XDNY236) （ZDYF2022XDNY236）
北京动物园圈养野生动物技术北京市重点实验室项目(ZDK202202) （ZDK202202）
