国内部分动物园与花鸟市场鹦鹉热衣原体病原学检测OA

In order to investigate the prevalence of Chlamydia psittaci(Cps)in bird flocks in some zoos and flower-bird markets in China,168 avian fecal samples collected from three sampling sites in Jiangsu Province and Beijing were detected using Taq Man fluorescence quantitative PCR,and gene sequence analysis was conducted for positive samples based on OmpA gene.The results showed that 31 Cps-positive samples were detected in a flower-bird market in Jiangsu Province,with an individual positive rate of 41.3%(31/75),but none was in the other two sampling sites(zoos);the positive samples were from budgerigars(23),peafowl parrots(5),gentoo parrots(2)and perching birds(1).For the positive samples,18 OmpA gene fragments were amplified,in which 16 samples had the same gene sequence,that were not in the same branch as the gene sequence in other two positive samples,but all of which belonged to genotype A.In conclusion,the risk of Cps prevalence in the flower-bird markets was relatively high,with concentrated sources of infection.Cps should be further prevented and controlled in local flower-bird markets,relevant regulatory measures should be implemented to improve quarantine supervision,aiming to reduce its transmission rate and safeguard public health safety.