中国兽医科学2024，Vol.54Issue(6)：757-765,9.DOI:10.16656/j.issn.1673-4696.2024.0113
冰晶调控剂对活疫苗半成品中伪狂犬病病毒效价的影响
Effect of ice crystal regulator on the titer of pseudorabies virus in semi-manufactured live vaccine
摘要
Abstract
In order to solve the problem that the titer of porcine pseudorabies virus(PRV)de-creased significantly after the semi-manufactured live virus vaccine was stored at-20 ℃,four kinds of ice crystal regulators with different concentrations were added to PRV suspension,and the titer loss before and after freezing and thawing was detected after five freeze-thaw cycles.The virus titer loss of 0.25 mol/L AMG,0.14 mol/L Suc,0.45 mmol/L PVP and 0.40 mol/L Lac groups were 0.20,0.26,0.25,0.31 lg TCID50/mL,respectively.0.25 mol/L AMG with the best protective effect was mixed with PRV suspension to prepare the semi-manufactured vaccine,and after 60 days of storage at-20 ℃,the virus titer loss was less than 0.6 lg TCID50/mL.The results of safety evaluation showed that 0.25 mol/L AMG had no obvious toxicity to mice and cells.The results showed that the semi-manufactured porcine PRV prepared by adding 0.25 mol/L AMG to the virus suspension after freeze-thaw cycle had little loss of titer,and was safe for mice and cells,which provided an important theoretical basis for the optimization of freeze-drying process of virus live vaccine production.关键词
伪狂犬病病毒/活疫苗/冰晶调控剂/冻干工艺优化Key words
pseudorabies virus/live vaccine/ice crystal regulator/optimization of freeze-drying process分类
农业科技引用本文复制引用
赵艳红,孟繁荣,吕芳,王军宁,左晓昕,揭鸿英,卢宇,鲍恩东..冰晶调控剂对活疫苗半成品中伪狂犬病病毒效价的影响[J].中国兽医科学,2024,54(6):757-765,9.基金项目
江苏省农业科技自主创新资金项目(CX(22)2016) （CX(22）
