冰晶调控剂对活疫苗半成品中伪狂犬病病毒效价的影响OA北大核心CSTPCD

In order to solve the problem that the titer of porcine pseudorabies virus(PRV)de-creased significantly after the semi-manufactured live virus vaccine was stored at-20 ℃,four kinds of ice crystal regulators with different concentrations were added to PRV suspension,and the titer loss before and after freezing and thawing was detected after five freeze-thaw cycles.The virus titer loss of 0.25 mol/L AMG,0.14 mol/L Suc,0.45 mmol/L PVP and 0.40 mol/L Lac groups were 0.20,0.26,0.25,0.31 lg TCID50/mL,respectively.0.25 mol/L AMG with the best protective effect was mixed with PRV suspension to prepare the semi-manufactured vaccine,and after 60 days of storage at-20 ℃,the virus titer loss was less than 0.6 lg TCID50/mL.The results of safety evaluation showed that 0.25 mol/L AMG had no obvious toxicity to mice and cells.The results showed that the semi-manufactured porcine PRV prepared by adding 0.25 mol/L AMG to the virus suspension after freeze-thaw cycle had little loss of titer,and was safe for mice and cells,which provided an important theoretical basis for the optimization of freeze-drying process of virus live vaccine production.