中国药理学与毒理学杂志2024，Vol.38Issue(6)：420-425,6.DOI:10.3867/j.issn.1000-3002.2024.06.003
铁硫簇结合蛋白1的磁感应能力
Ability of iron sulfur cluster binding protein 1 to respond to magnetic fields
摘要
Abstract
OBJECTIVE To explore the effect of iron sulfur cluster binding protein 1(ISCA1)mag-netic field on calcium inflow.METHODS ① A plasmid containing the ISCA1、Magneto 2.0 gene sequence,and an empty plasmid was prepared for lentivirus packaging,with all the three plasmids carrying the mCherry gene.The infection efficiency of lentiviruses was assessed using fluorescence microscopy.② The immunoprecipitation technique was employed to ascertain the interactions between ISCA1 and cryptochrome(CRY1)as well as CRY2 proteins.③ The technique of live cell calcium imaging was used to detect the intracellular calcium influx in cells exhibiting high levels of ISCA1 or Magneto 2.0 expression under the influence of a magnetic field(40 mT,0.1Hz,90%duty cycle).RESULTS ① Red fluorescence was observed by fluorescence microscopy,indicating successful transfection of lentivirus.② The exogenous ISCA1 proteins exhibited no affinity towards the endogenous CRY1 or CRY2 proteins.③ The green fluorescence intensity of the Magneto 2.0 group increased by(1.8±0.5)times compared to the pre-magnetic addition level,indicating a significant influx of calcium ions.However,there was no significant difference in the green fluorescence intensity between the ISCA1 group and control group before and after magnetic addition.CONCLUSION Cells with exogenously high expres-sion of ISCA1 do not elicit calcium influx and exhibit no discernible magnetic induction following stimula-tion by this magnetic field.关键词
铁硫簇结合蛋白1/隐花色素/磁受体蛋白/磁感应/磁遗传学Key words
iron sulfur cluster binding protein 1/cryptochrome/magnetic receptor protein/magnet-oception/magnetogenetics分类
医药卫生引用本文复制引用
刘梦楠,田萧羽,方文灿,吴宁,李锦,李红..铁硫簇结合蛋白1的磁感应能力[J].中国药理学与毒理学杂志,2024,38(6):420-425,6.基金项目
国家自然科学基金(82373853) （82373853）
国家自然科学基金(82202523) National Natural Science Foundation of China(82373853) （82202523）
and National Natural Science Foundation of China(82202523) （82202523）
