肿瘤防治研究2024，Vol.51Issue(6)：426-431,6.DOI:10.3971/j.issn.1000-8578.2024.23.1380
新型重组溶瘤痘苗病毒感染联合免疫效应细胞及免疫检查点阻断三联治疗胃癌的研究
Experimental Study on Treatment of Gastric Cancer with New Recombinant Oncolytic Vaccinia Virus Infection Combined with Immune Effective Cells and Immune Checkpoint Blocking
摘要
Abstract
Objective To explore the significance of the combined strategy of oncolytic virus infection,immune effector cell supplement,and immune checkpoint blocking in the treatment of gastric cancer.Methods A tumor-bearing nude mouse model of gastric cancer was treated with recombinant oncolytic vaccinia virus carrying the CXCL9 gene of T lymphocyte chemokine and IL-7gene(VV-IL7-CXCL9)obtained in previous experiments.We established a triple-intervention group of recombinant oncolytic vaccinia virus intratumoral injection+cytotoxic T lymphocyte(CTL)+immune checkpoint blocker(PD-1 monoclonal antibody),a single-intervention group of three measures,a dual-intervention group,and a blank control group.After the intervention,tumor-growth curve method,tumor-inhibition rate method,and bioluminescence method were used to detect the tumor treatment effect.ELISA was used to detect CXCL9 and IL-7 molecule concentration in the serum and tissue homogenate of animals in each group.Results The therapeutic results of animal models showed that the tumor growth in the triple-intervention group of recombinant oncolytic poxvirus+CTL+immune checkpoint blocker(PD-1 monoclonal antibody)was significantly slower than those in the other intervention and the blank control group.Conclusion The combination of recombinant oncolytic poxvirus intratumoral injection+CTL+immune checkpoint blocker(PD-1 monoclonal antibody)exert a strong antitumor effect in intervention experiments on gastric-cancer animal models.关键词
胃癌/溶瘤痘病毒/免疫效应细胞/免疫检查点阻断治疗Key words
Gastric cancer/Oncolytic poxvirus/Immune effector cells/Immune checkpoint blocking therapy分类
医药卫生引用本文复制引用
张林,侯艳红,吴凯,张静..新型重组溶瘤痘苗病毒感染联合免疫效应细胞及免疫检查点阻断三联治疗胃癌的研究[J].肿瘤防治研究,2024,51(6):426-431,6.基金项目
Capital Health Development Research Special Fund(No.2011-5007-01) 首都卫生发展科研专项基金(2011-5007-01) （No.2011-5007-01）
