Considerations for the use of porcine organ donation models in preclinical organ donor intervention researchOA
Use of animal models in preclinical transplant research is essential to the optimization of human allografts for clinical transplantation.Animal models of organ donation and preservation help to advance and improve technical elements of solid organ recovery and facilitate research of ischemia-reperfusion injury,organ preservation strategies,and future donor-based interventions.Important considerations include cost,public opinion regarding the conduct of animal research,translational value,and relevance of the animal model for clinical practice.We present an overview of two porcine models of organ donation:donation following brain death(DBD)and donation following circulatory death(DCD).The cardiovascular anatomy and physiology of pigs closely resembles those of humans,making this species the most appropriate for pre-clinical research.Pigs are also considered a potential source of organs for human heart and kidney xenotransplantation.It is imperative to minimize animal loss during procedures that are surgically complex.We present our experience with these models and describe in detail the use cases,procedural approach,challenges,alternatives,and limitations of each model.
Frazer I.Heinis;Shaheed Merani;Nicholas W.Markin;Kim F.Duncan;Michael J.Moulton;Lance Fristoe;William E.Thorell;Raechel A.Sherrick;Tami R.Wells;Matthew T.Andrews;Marian Urban;
School of Natural Resources,Institute of Agriculture and Natural Resources,University of Nebraska-Lincoln,Lincoln,Nebraska,USADivision of Transplantation and Vascular Surgery,Department of Surgery,University of Nebraska Medical Center,Omaha,Nebraska,USADepartment of Anesthesiology,University of Nebraska Medical Center,Omaha,Nebraska,USADivision of Cardiothoracic Surgery,Department of Surgery,University of Nebraska Medical Center,Omaha,Nebraska,USAClinical Perfusion,Nebraska Medicine-Nebraska Medical Center,Omaha,Nebraska,USADepartment of Neurosurgery,University of Nebraska Medical Center,Omaha,Nebraska,USANutrition and Health Sciences,College of Education and Human Sciences,University of Nebraska-Lincoln,Lincoln,Nebraska,USADepartment of Comparative Medicine,University of Nebraska Medical Center,Omaha,Nebraska,USA
临床医学
animal modelbrain deathcirculatory deathorgan transplantation
《Animal Models and Experimental Medicine》 2024 (003)
P.283-296 / 14
University of Nebraska Collaborative Initiative,Grant/Award Number:Team Seed Grant#26685。
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