解磷菌与解磷固氮双效菌对贵州富硒土及茶苗硒锌含量的影响OA北大核心CSTPCD

In order to investigate the effects of phosphate solubilizing bacteria and phosphate-solubilizing&nitrogen-fixing bacteria on the content of available selenium(Se)and zinc(Zn)in selenium-rich soil and Camellia sinensis,two strains of endogenous phosphate solubilizing bacteria(Paraburkholderia fungorum PSt07,Kluyvera intermedia PSt12)and two endogenous phosphate-solubilizing&nitrogen-fixation bacteria(Paraburkholderia fungorum PMS05,Kluyvera intermedia PCF06)were used as the research object,and 2 year old tea seedlings of'Longjing43'and'Huangjinya'and Se-rich red soil of Kaiyang County,Guizhou Province were used as the test materials.Soil incubation and pot experiment of tea seedlings were carried out.After 60 days of inoculation with each bacterial solution,Se and Zn contents in tea seedlings and available nutrients of nitrogen(N),phosphorus(P),potassium(K),Zn,Se in soil were tested.The results show that the tested strains could increase the content of available P in rhizosphere soil of two tea seedlings,and the content of available N in rhizosphere soil of'Longjing43'.Under the treatment of phosphate solubilizing bacteria,the content of available Se and Zn in rhizosphere soil and Se in root tissue of'Longjing43'tea seedlings were increased by 191.83%-573.08%,37.48%-65.88%and 24.27%-39.73%respectively.The accumulation of Zn in the leaves of the two tea seedlings was increased by 41.23%-247.65%(P<0.05).The contents of available Zn in the rhizosphere soil of potted plants under the treatment of two strains of phosphate solubilizing bacteria were higher than those in the soil incubated with bacteria solution alone without tea plants,indicating that the combined effect of tea plants and phosphate solubilizing bacteria could significantly improve the availability of Zn in soil.The phosphate-solubilizing&nitrogen-fixing bacteria were more beneficial than the phosphate solubilizing bacteria to increase the Se content of'Huangjinya'root tissue and the available Se content of rhizosphere soil,and PMS05 could significantly increase the content of available Se in rhizosphere soil by 602.00%(P<0.05).The accumulation of Se and Zn elements in tea varied greatly from different combinations of strains and tea cultivars.In practical application,the optimal phosphorus solubilizing strain should be selected for different tea cultivars after completing the strain selection and fertilizer efficiency verification experiment.