茶叶科学2024，Vol.44Issue(3)：443-452,10.
贝莱斯芽孢杆菌YJK1鉴定及其对茶炭疽病的拮抗效果
Antagonistic Activity and Utilization of Bacillus velezensis Strain YJK1 as A Biocontrol Agent Against Anthracnose on Camellia sinensis
摘要
Abstract
The tea industry is one of China's important agricultural economic resources.However,widespread outbreaks of anthracnose on Camellia sinensis pose a significant threat to the sustainability of the tea industry.Conventional approaches based on chemical pesticides face inherent challenges,including pesticide residue accumulation and environmental pollution.In this study,the biological control effect of Bacillus velezensis YJK1 on tea tree diseases was evaluated.The results demonstrat that the YJK1 isolate exhibited robust antagonistic activity against fungal diseases affecting tea trees,particularly against pathogens such as Colletotrichum camelliae,Colletotrichum fructicola and Fusarium fujikuroi.YJK1 fermentation diluent has a significant inhibitory effect on the spores of C.camelliae and C.fructicola.The inhibitory effects of YJK1 fermented liquid on the diameter of C.camelliae were evaluated with the detached tea leaves.YJK1 fermented liquid effectively prevented tea tree anthracnose with 78.1%control when applied 24 h before C.camelliae inoculation and 61.8%during simultaneous inoculation.However,its efficacy was limited(post-inoculation).The YJK1 fermentation broth has a broad-spectrum of activity against various fungi.The antagonistic activity of the fermented liquid was stable below 80℃.After 60 min of UV treatment,the inhibition rate was still above 50%.And after treatment with different pH values,there was no significant difference in the inhibition rate within the pH range of 5-11.These findings suggest that B.velezensis YJK1 is a promising biocontrol agent for the management of tea plant diseases.关键词
贝莱斯芽孢杆菌/茶炭疽病/拮抗菌/生物防治/稳定性Key words
Bacillus velezensis/tea anthracnose/antagonistic bacteria/biological control/stability分类
农业科技引用本文复制引用
唐朝阳,闵莉静,孔丽娅,胡骞,宋秋瑾,何鲁钱,楼骏,王占旗,何艳,张立钦..贝莱斯芽孢杆菌YJK1鉴定及其对茶炭疽病的拮抗效果[J].茶叶科学,2024,44(3):443-452,10.基金项目
国家自然科学基金(42107019)、国家级大学生创新创业训练计划项目(202310347056)、湖州市自然科学资金项目(2022YZ11)、浙江省教育厅研究项目(Y202248499)、浙江省重点研发计划尖兵项目(2023C02004) （42107019）
