南方农业学报2024，Vol.55Issue(4)：1035-1045,11.DOI:10.3969/j.issn.2095-1191.2024.04.012
三七叶际微生物的分离鉴定及生防功能研究
Isolation and identification of phyllosphere microbiome of Panax notoginseng(Burk.)F.H.Chen and its biocontrol functions
摘要
Abstract
[Objective]The purpose of the study was to isolate and screen biocontrol strains from Panax notoginseng(Burk.)F.H.Chen phyllosphere with strong inhibition effect against black spot disease of P.notoginseng leaves,in order to provide microbial resources for the development of safe and efficient biocontrol fungicides,as well as to provide reference for the ecological management of diseases affecting Chinese medicinal materials.[Method]Cultivable phyllosphere micro-biomes were isolated from healthy one-year-old P.notoginseng leaves using serial dilution method.The antagonistic effects of the isolated strains against Alternaria panax strain SL17,a pathogen causing black spot disease on P.notogin-seng leaves,were evaluated using plate confrontation method.Phyllosphere microbiome strains with biocontrol effects against A.panax were screened.The active strains were classified and identified by ITS and 16S rDNA sequencing,and the strains with the best inhibition effects were further selected for the determination of broad-spectrum inhibition ability.[Result]A total of 125 isolates were isolated and purified from healthy P.notoginseng phyllosphere,from which 50 an-tagonistic strains with obvious inhibition effects against A.panax were obtained using plate confrontation method,inclu-ding 26 fungal strains and 24 bacterial strains.Among the 26 antagonistic fungi,two P.notoginseng leaf endophytic fungal strains NZ-14 and NZ-23 showed the best inhibitory effects,of which Trichoderma koningii NZ-14 could completely in-hibit the mycelial growth of A.panax with an inhibition rate of 100.00% .Among the 24 antagonistic bacterial isolates,two strains of P.notoginseng leaf endophytic bacteria NX-20 and NX-23 had the best inhibition effect,with Bacillus subti-lis NX-20 having an inhibition rate of 71.13% against A.panax.The results of the inhibitory activity assays of the phyllo-sphere antagonists against the main fungal pathogens of P.notoginseng showed that the strains NZ-14,NZ-23,NX-20 and NX-23 had good inhibition activity against Mycocentrospora acerina strain DMS5,Fusarium solani strain F3,and Cylindrocarpon destructans strain RS006,with inhibition rates ranging from 39.26% to 78.97% .[Conclusion]There are many microbiomes in the phyllosphere of P.notoginseng with potential inhibition activity,among which the two Tricho-derma strains NZ-14 and NZ-23 and the two Bacillus strains NX-20 and NX-23 have the best inhibition effects and strong inhibitory activity against the main fungal pathogens of P.notoginseng leaves and roots,and can be used as microbial re-sources for biological control of P.notoginseng diseases.关键词
三七/叶际微生物/黑斑病/拮抗菌/木霉菌/芽孢杆菌/生物功能Key words
Panax notoginseng(Burk.)F.H.Chen/phyllosphere microbiome/black spot/antagonistic bacteria/Trichoderma/Bacillus/biological function分类
农业科技引用本文复制引用
禄蓉,张秀,田光祥,陈朝苍,赵云思,朱书生,杨敏..三七叶际微生物的分离鉴定及生防功能研究[J].南方农业学报,2024,55(4):1035-1045,11.基金项目
国家自然科学基金项目(32060719) （32060719）
云南省农林联合重点项目(202101BD070001-003) （202101BD070001-003）
云南省中青年后备人才项目(202005AC160045) National Natural Science Foundation of China(32060719) （202005AC160045）
Yunnan Agriculture and Forestry Joint Key Project(202101BD070001-003) （202101BD070001-003）
Yunnan Young and Middle-aged Reserve Talent Project(202005AC160045) （202005AC160045）
