MWCNTs-COOH对干旱胁迫下小麦幼苗生长的影响OA北大核心CSTPCD
Effect of MWCNTs-COOH on Growth of Wheat Seedlings under Drought Stress
采用10%PEG模拟干旱胁迫,以超纯水和10%PEG溶液处理为非干旱处理对照(CK1)和干旱处理对照(CK2),研究不同质量浓度羧基化多壁碳纳米管(MWCNTs-COOH,10、20、40、80、160、320 mg/L)对干旱胁迫下小麦幼苗生长的影响,为缓解干旱胁迫对小麦的伤害提供理论基础和新的途径.结果表明,干旱胁迫下,随着MWCNTs-COOH质量浓度增加,小麦苗高、地上部鲜质量、地上部干质量、最大根长、根鲜质量、根干质量均呈先升高后降低的趋势,以160 mg/L MWCNTs-COOH处理最高.与CK2相比,10～160 mg/L MWCNTs-COOH处理提高了干旱胁迫下小麦苗高、最大根长、地上部鲜质量、地上部干质量、根鲜质量、根干质量,促进小麦生长,且对根的促进作用高于地上部;320 mg/L MWCNTs-COOH处理降低了干旱胁迫下苗高、地上部鲜质量、地上部干质量,抑制小麦生长.对小麦叶片生理生化指标和根系活力进行分析发现,与CK2相比,160 mg/L MWCNTs-COOH处理小麦叶片含水量和相对含水量提高,水分饱和亏和丙二醛(MDA)含量降低,脯氨酸含量、抗氧化酶活性增加,根系活力增强,高质量浓度的MWCNTs-COOH(320 mg/L)则使各指标值向相反方向发展,胁迫程度加重.表明低质量浓度MWCNTs-COOH处理可通过保持叶片水分和激活植物防御系统,诱导幼苗对干旱产生耐受性.综上,适宜质量浓度(10～160 mg/L)的MWCNTs-COOH可促进干旱胁迫下小麦幼苗生长,增强其抗旱性,以160 mg/L MWCNTs-COOH处理效果最好;高质量浓度(320 mg/L)的MWCNTs-COOH对干旱胁迫下小麦幼苗具有毒性效应.
10%PEG was used to simulate drought stress,ultrapure water and 10%PEG solution treatments were used as non-drought treatment control(CK1)and drought treatment control(CK2),and the effects of different concentrations of carboxylated multiwalled carbon nanotubes(MWCNTs-COOH;10,20,40,80,160,320 mg/L)on the growth of wheat seedlings under drought stress were studied,so as to provide a theoretical basis and new approach for alleviating wheat drought stress.The results showed that with the increase of MWCNTs-COOH concentration,the wheat seedling height,fresh shoot weight,dry shoot weight,maximum root length,fresh root weight and dry root weight all first increased and then decreased under drought stress.The highest values were observed in the treatment with 160 mg/L MWCNTs-COOH.Compared with CK2,the treatment of 10-160 mg/L MWCNTs-COOH increased wheat seedling height,maximum root length,fresh shoot weight,dry shoot weight,fresh root weight and dry root weight under drought stress,promoting wheat growth,and the promoting effect on roots was higher than that on shoots;320 mg/L MWCNTs-COOH treatment reduced seedling height,fresh shoot weight and dry shoot weight under drought stress,and inhibited wheat growth.Analysis of physiological and biochemical indicators of wheat leaves and root activity showed that compared with CK2,the treatment with 160 mg/L MWCNTs-COOH increased the water content,relative water content,proline content and antioxidant enzyme activity of wheat leaves,decreased water saturation deficit and malonaldehyde content,and enhanced root activity.High concentration of MWCNTs-COOH(320 mg/L)led to the opposite development of various indicator values,and increased stress levels.The results indicated that low concentration of MWCNTs-COOH treatment could induce drought tolerance of seedlings by maintaining leaf water content and activating plant defense systems.In summary,appropriate concentrations of MWCNTs-COOH(10-160 mg/L)can promote the growth of wheat seedlings under drought stress,enhance drought resistance,and 160 mg/L MWCNTs-COOH treatment has the best effect;High concentration of MWCNTs-COOH(320 mg/L)has toxic effects on wheat seedlings under drought stress.
韩金玲;钟婷玮;杨晴;赵晓丽;杨敏;王秀平
河北科技师范学院 农学与生物科技学院,河北 秦皇岛 066000||河北省作物逆境生物学重点实验室,河北 秦皇岛 066000河北科技师范学院 农学与生物科技学院,河北 秦皇岛 066000河北农信生物科技有限责任公司,河北 石家庄 050000河北科技师范学院 分析测试中心,河北 秦皇岛 066000
农业科学
小麦干旱胁迫羧基化多壁碳纳米管生长
WheatDrought stressMWCNTs-COOHGrowth
《河南农业科学》 2024 (006)
18-26 / 9
河北省重点研发计划项目(21326507D)
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