外源6-BA对灌浆期谷子光合特性、抗氧化系统及产量的影响OA北大核心CSTPCD
Effects of Exogenous 6-BA on Photosynthesis,Antioxidant System Characteristics,and Yield-Related Traits in Foxtail Millet at Grain Filling Stage
为探究外源细胞分裂素对灌浆期谷子(Setaria italica)光合特性、抗氧化系统及产量的影响,以张杂谷5号和晋谷21号为试验材料,在灌浆期分别喷施0(对照)、2.5、5、10、20和40 mg·L-1的6-苄氨基嘌呤(6-BA),分析外源6-BA对谷子叶绿素荧光及光合特性、抗氧化特性、内源激素含量和产量性状的影响.结果表明,张杂谷5号和晋谷21号叶片的净光合速率(Pn)、蒸腾速率(Tr)、气孔导度(Gs)、胞间二氧化碳浓度(Ci)和电子传递速率(ETR)均随6-BA喷施浓度的增加呈先升高后降低趋势,以10 mg·L-1处理增幅最大,分别较对照增加11.46%、6.02%、5.56%、7.11%、9.41%和12.08%、9.96%、8.21%、12.76%、14.87%;而非光化学猝灭(NPQ)的变化呈相反趋势.此外,随着6-BA喷施浓度的增加,两品种叶片过氧化物酶、超氧化物歧化酶活性和可溶性糖含量及成熟期产量和产量构成因素也呈先升高后降低趋势,以10 mg·L-1处理两品种的产量增幅最大,分别较对照提高7.08%和8.89%;丙二醛含量则先降低后升高.进一步分析10 mg·L-1 处理下内源激素变化发现,两品种叶片的玉米素含量分别较对照提高75.64%和80.71%,脱落酸含量分别降低37.50%和6.06%.相关性分析结果表明,叶绿素a、净光合速率和电子传递速率是影响谷子产量性状的主要因子.可见,外源6-BA可延缓叶片衰老,提升光合速率,有利于碳的固定,是提高谷子产量的有效途径.综上,谷子灌浆期喷施6-BA的最佳浓度为10 mg·L-1.本研究结果为细胞分裂素在谷子中的高效利用提供了依据.
To explore the effects of the cytokinin on the photosynthetic characteristics,antioxidant system characteristics,and yield-related traits of foxtail millet at grain filling stage,two foxtail millet varieties(Zhangzagu 5 and Jingu 21)were sprayed with six concentrations of 6-BA viz.0,2.5,5,10,20,and 40 mg·L-1 during the grain-filling stage in field experiment.In compared with control,net photosynthetic rate(Pn),transpiration rate(Tr),stomatal conductance(Gs),intercellular carbon dioxide concentration(Ci),and electron transfer rate(ETR)in foxtail millet leaves,exhibited an initial increased followed by a subsequent decreased as the spraying concentration increasing.The maximum facilitation effect of the treatments was observed at a concentration of 10 mg·L-1 of 6-BA,which increased on average by 11.46%,6.02%,5.56%,7.11%,9.41%in Zhangzagu 5 and 12.08%,9.96%,8.21%,12.76%,14.87%in Jingu 21,respectively.At the same time,peroxidase,superoxide,soluble sugar content of antioxidant properties,yield and yield-related traits showed the similar phenomenon with the spraying concentration increased.Moreover,the change tendency of non-photochemical quenching(NPQ)and malondialdehyde were opposite.Under the treatment of 10 mg·L-1 6-BA,the average yield of Zhangzagu 5 and Jingu 21 exhibited an increase of 7.08%and 8.89%,respectively,in comparison with control.Further analysis showed that the application of 10 mg·L-1 6-BA resulted in a significant increase of zeatin(Zt)levels by 75.64%and 80.71%and led to a notable reduction in abscisic acid(ABA)levels by 37.50%and 6.06%in Zhangzagu 5 and Jingu 21,respectively.Correlation analysis showed that chlorophyll-a,Pn,and ETR were the main factors affected yield-related traits with exogenous cytokinin.These results also indicated that exogenous cytokinin application delayed leaf senescence and improved photochemical utilization,which facilitated the fixation and reduction of carbon,ultimately increased grain yield in foxtail millet.In summary,it is recommended to use 10 mg·L-1 6-BA in field,which is best effect for foxtail millet.The results provide the basis for the efficient utilization of cytokinin in millet.
孟颖超;李伟;孙瑶;冯志威;王娟玲
山西农业大学棉花研究所,山西 运城 044000
谷子6-BA产量光合特性抗氧化酶活性
foxtail millet6-BAyieldphotosynthetic characteristicsantioxidant enzyme activity
《核农学报》 2024 (008)
1605-1615 / 11
山西省科技重大专项计划"揭榜挂帅"项目(202101140601026-6-10)
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