核农学报2024，Vol.38Issue(8)：1605-1615,11.DOI:10.11869/j.issn.1000-8551.2024.08.1605
外源6-BA对灌浆期谷子光合特性、抗氧化系统及产量的影响
Effects of Exogenous 6-BA on Photosynthesis,Antioxidant System Characteristics,and Yield-Related Traits in Foxtail Millet at Grain Filling Stage
摘要
Abstract
To explore the effects of the cytokinin on the photosynthetic characteristics,antioxidant system characteristics,and yield-related traits of foxtail millet at grain filling stage,two foxtail millet varieties(Zhangzagu 5 and Jingu 21)were sprayed with six concentrations of 6-BA viz.0,2.5,5,10,20,and 40 mg·L-1 during the grain-filling stage in field experiment.In compared with control,net photosynthetic rate(Pn),transpiration rate(Tr),stomatal conductance(Gs),intercellular carbon dioxide concentration(Ci),and electron transfer rate(ETR)in foxtail millet leaves,exhibited an initial increased followed by a subsequent decreased as the spraying concentration increasing.The maximum facilitation effect of the treatments was observed at a concentration of 10 mg·L-1 of 6-BA,which increased on average by 11.46%,6.02%,5.56%,7.11%,9.41%in Zhangzagu 5 and 12.08%,9.96%,8.21%,12.76%,14.87%in Jingu 21,respectively.At the same time,peroxidase,superoxide,soluble sugar content of antioxidant properties,yield and yield-related traits showed the similar phenomenon with the spraying concentration increased.Moreover,the change tendency of non-photochemical quenching(NPQ)and malondialdehyde were opposite.Under the treatment of 10 mg·L-1 6-BA,the average yield of Zhangzagu 5 and Jingu 21 exhibited an increase of 7.08%and 8.89%,respectively,in comparison with control.Further analysis showed that the application of 10 mg·L-1 6-BA resulted in a significant increase of zeatin(Zt)levels by 75.64%and 80.71%and led to a notable reduction in abscisic acid(ABA)levels by 37.50%and 6.06%in Zhangzagu 5 and Jingu 21,respectively.Correlation analysis showed that chlorophyll-a,Pn,and ETR were the main factors affected yield-related traits with exogenous cytokinin.These results also indicated that exogenous cytokinin application delayed leaf senescence and improved photochemical utilization,which facilitated the fixation and reduction of carbon,ultimately increased grain yield in foxtail millet.In summary,it is recommended to use 10 mg·L-1 6-BA in field,which is best effect for foxtail millet.The results provide the basis for the efficient utilization of cytokinin in millet.关键词
谷子/6-BA/产量/光合特性/抗氧化酶活性Key words
foxtail millet/6-BA/yield/photosynthetic characteristics/antioxidant enzyme activity引用本文复制引用
孟颖超,李伟,孙瑶,冯志威,王娟玲..外源6-BA对灌浆期谷子光合特性、抗氧化系统及产量的影响[J].核农学报,2024,38(8):1605-1615,11.基金项目
山西省科技重大专项计划"揭榜挂帅"项目(202101140601026-6-10) （202101140601026-6-10）
